ppargc1asa13186 heterozygous incrosses were incubated in 0.003% phenylthiourea (Sigma, P7629) in E3 from 24 hpf until 48 hpf. Zebrafish were anesthetized in 0.02% tricaine and microinjected was performed directly into the circulation as described (Kroeger et al., 2017 (link)) at the 48 hpf time point with 5 mg/ml 40 kDa dextran-FITC (Invitrogen, D1845). Embryos were again incubated in 0.003% PTU until 72 hpf when they were imaged.
40 kda dextran fitc
Manufactured by Thermo Fisher Scientific
40 kDa dextran-FITC is a fluorescently labeled polysaccharide compound with a molecular weight of 40 kilodaltons. It is used as a tool in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 40 kda dextran fitc
1
Dextran-FITC Vascular Labeling in Zebrafish
2
Dextran-FITC Vascular Labeling in Zebrafish
Check if the same lab product or an alternative is used in the 5 most similar protocols
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ppargc1asa13186 heterozygous incrosses were incubated in 0.003% phenylthiourea (Sigma, P7629) in E3 from 24 hpf until 48 hpf. Zebrafish were anesthetized in 0.02% tricaine and microinjected was performed directly into the circulation as described (Kroeger et al., 2017 (link)) at the 48 hpf time point with 5 mg/ml 40 kDa dextran-FITC (Invitrogen, D1845). Embryos were again incubated in 0.003% PTU until 72 hpf when they were imaged.
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