Log In Sign up
The largest database of trusted experimental protocols

Lumikine xpress mifn β 2

Manufactured by InvivoGen
Visit Supplier

The LumiKine™ Xpress mIFN-β 2.0 is a sensitive and quantitative bioluminescent assay for the detection of mouse interferon-beta (mIFN-β) in cell culture supernatants. The assay utilizes a reporter cell line that expresses the luciferase gene under the control of the mIFN-β promoter, providing a direct measurement of mIFN-β levels.

Automatically generated - may contain errors

Lab products found in correlation

2 3 cgamp elisa kit, by Cayman Chemical (1 mentions) M per lysis buffer, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

LumiKine (4 citations) LumiKine™ Xpress mIFN-β 2.0 kit (2 citations)

4 protocols using lumikine xpress mifn β 2

1

STING Agonist Induces IFN-β Response

Check if the same lab product or an alternative is used in the 5 most similar protocols
Serum was obtained 4 h after the first dose of STING agonist. ELISA was performed for IFN-β using LumiKine Xpress mIFN-β 2.0 (InvivoGen) according to the manufacturer’s instructions.
Sokolowska O., Rodziewicz-Lurzynska A., Pilch Z., Kedzierska H., Chlebowska-Tuz J., Sosnowska A., Szumera-Cieckiewicz A., Sokol K., Barankiewicz J., Salomon-Perzynski A., Ciepiela O., Lech-Maranda E., Golab J, & Nowis D. (2022). Immune checkpoint inhibition improves antimyeloma activity of bortezomib and STING agonist combination in Vk*MYC preclinical model. Clinical and Experimental Medicine, 23(5), 1563-1572.
+ Open protocol
+ Expand
2

SARS-CoV-2 Infection Induces IFNβ and cGAMP Production

Check if the same lab product or an alternative is used in the 5 most similar protocols
Calu3 cells infected with SARS-CoV-2 were collected with mPER lysis buffer (Thermo Fisher) and inactivated SARS-CoV-2 followed with UV radiation. The production of IFNβ in cells was measured by LumiKine™ Xpress mIFN-β 2.0 (Invivogen) was used according to the manufacturer instructions. The production of cGAMP in cells was measured by 2'3’-cGAMP ELISA Kit (Cayman Chemical) according to the manufacturer instructions. Detailed product catalogs are listed in Supplementary Table 1.
Cai S., Zhang C., Zhuang Z., Zhang S., Ma L., Yang S., Zhou T., Wang Z., Xie W., Jin S., Zhao J., Guan X., Wu J., Cui J, & Wu Y. (2023). Phase-separated nucleocapsid protein of SARS-CoV-2 suppresses cGAS-DNA recognition by disrupting cGAS-G3BP1 complex. Signal Transduction and Targeted Therapy, 8, 170.
+ Open protocol
+ Expand
3

Quantifying mIFNβ Levels Post-cGAMP/STING-NP

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasma concentration of mIFNβ at 1, 4, 8, 24, and 48 h post-injection of cGAMP or STING-NPs was quantified using the LumiKine™ Xpress mIFN-β 2.0 (Invivogen) as per manufacturer’s instructions.
Wehbe M., Wang-Bishop L., Becker K.W., Shae D., Baljon J.J., He X., Christov P., Boyd K.L., Balko J.M, & Wilson J.T. (2020). Nanoparticle Delivery Improves the Pharmacokinetic Properties of Cyclic Dinucleotide STING Agonists to Open a Therapeutic Window for Intravenous Administration. Journal of controlled release : official journal of the Controlled Release Society, 330, 1118-1129.
+ Open protocol
+ Expand
4

Detecting Cytokine Responses to Lm Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols
DCs were cultured treated or not with DEVs overnight, supernatant was collected and frozen at -80°C for ELISA analysis. IL-1β assays were performed following the manufacturer instructions with undiluted DCs supernatant (88-7013-88, Invitrogen). Whole cell lysates and DEVs from HDAC6-WT and KO, infected or not with Lm were analyzed by LumiKine™ Xpress mIFN-β 2.0 (InvivoGen, luex-mifnbv2a) following manufacture instructions.
Izquierdo-Serrano R., Fernández-Delgado I., Moreno-Gonzalo O., Martín-Gayo E., Calzada-Fraile D., Ramírez-Huesca M., Jorge I., Camafeita E., Abián J., Vicente-Manzanares M., Veiga E., Vázquez J, & Sánchez-Madrid F. (2022). Extracellular vesicles from Listeria monocytogenes-infected dendritic cells alert the innate immune response. Frontiers in Immunology, 13, 946358.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!