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Cell light edu apollo 567 imaging kit

Manufactured by RiboBio
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Sourced in China

The Cell-Light EdU Apollo 567 imaging kit is a laboratory equipment that enables the detection and visualization of newly synthesized DNA in cells. It utilizes the incorporation of EdU (5-ethynyl-2'-deoxyuridine), a thymidine analog, into the DNA of proliferating cells, which can then be detected using a fluorescent azide dye.

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Lab products found in correlation

Inverted fluorescence microscope, by Olympus (1 mentions) 2 4 amidinophenyl 6 indolecarbamidine dihydrochloride dapi, by Merck Group (1 mentions)

Spelling variants of this product

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2 protocols using cell light edu apollo 567 imaging kit

1

EdU Proliferation Assay for Cell Lines

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An EdU proliferation test was conducted using the in vitro Cell-Light EdU Apollo 567 imaging kit (RiboBio, Guangzhou, China). In brief, HCC cells were transfected with a plasmid or transduced with lentivirus and cultured for 48 h before being seeded in 96-well plates (2 × 104 cells/well) and cultured for another 24 h before EdU according to the manufacturer’s instructions. The figures of EdU-positive cells (red) in three random observation fields were captured via an inverted fluorescence microscope (Olympus, Beijing, China).
Zhang J., Ren Z., Zheng D., Song Z., Lin J., Luo Y., Zou X., Pan Y., Qi N., Li A, & Liu X. (2022). AHSA1 Promotes Proliferation and EMT by Regulating ERK/CALD1 Axis in Hepatocellular Carcinoma. Cancers, 14(19), 4600.
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2

Cell Proliferation Assay with EDU Kit

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Cell-Light™ EDU Apollo®567 Imaging Kit (Ribobio) was utilized to analyze cell proliferation ability. Transfected RASFs were incubated with 50 μM EDU reagent for 2 h. The nucleus of RASFs was marked with 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride (DAPI) (Sigma). Cell images were taken under a fluorescence microscope.
Yu H., Yang J., Chen K., Kang W, & Zhu F. (2023). Circ_0000396 suppresses the proliferation and inflammation of rheumatoid arthritis synovial fibroblasts by targeting miR-574-5p/RSPO1 axis. Journal of Orthopaedic Surgery and Research, 18, 718.
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