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Gridion flow cells

Manufactured by Oxford Nanopore
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Sourced in United Kingdom

The GridION flow cells are a key component of the GridION system, which is a modular, scalable platform for DNA and RNA sequencing. The flow cells provide a controlled environment for the nanopore-based sequencing process, allowing the passage of nucleic acid molecules through nanopores for real-time detection and analysis.

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Lab products found in correlation

Gridion mk1 device, by Oxford Nanopore (2 mentions) Ligation sequencing kit, by Oxford Nanopore (2 mentions) Native barcoding kit, by Oxford Nanopore (2 mentions)

Spelling variants of this product

GridION (42 citations) GridION with the R9.4.1 flow cells (2 citations)

2 protocols using gridion flow cells

1

Nanopore Sequencing of Amplicon Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sequencing libraries were prepared from 32 amplicon samples using the Native Barcoding Kit (Oxford Nanopore Technologies; cat. no. EXP-NBD104) paired with the Ligation Sequencing Kit (Oxford Nanopore Technologies; cat. no. SQK-LSK109) according to the manufacturer’s directions. Between five and eight sequencing libraries per flow cell were pooled for sequencing on five GridION flow cells (Oxford Nanopore Technologies; R9.4.1) for 72 hours on a GridION Mk1 device (Oxford Nanopore Technologies; Oxford, England, UK). Raw data was live basecalled using the high accuracy model in Guppy (v.3.2.10).
Goike J., Hsieh C.L., Horton A., Gardner E.C., Bartzoka F., Wang N., Javanmardi K., Herbert A., Abbassi S., Renberg R., Johanson M.J., Cardona J.A., Segall-Shapiro T., Zhou L., Nissly R.H., Gontu A., Byrom M., Maranhao A.C., Battenhouse A.M., Gejji V., Soto-Sierra L., Foster E.R., Woodard S.L., Nikolov Z.L., Lavinder J., Voss W.N., Annapareddy A., Ippolito G.C., Ellington A.D., Marcotte E.M., Finkelstein I.J., Hughes R.A., Musser J.M., Kuchipudi S.V., Kapur V., Georgiou G., Dye J.M., Boutz D.R., McLellan J.S, & Gollihar J.D. (2021). Synthetic repertoires derived from convalescent COVID-19 patients enable discovery of SARS-CoV-2 neutralizing antibodies and a novel quaternary binding modality. bioRxiv.
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2

Nanopore Sequencing of 16 Amplicon Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sequencing libraries were prepared from 16 amplicon samples using the Native Barcoding Kit (Oxford Nanopore Technologies; Cat. No. EXP-NBD104) paired with the Ligation Sequencing Kit (Oxford Nanopore Technologies; Cat. No. SQK-LSK109) according to the manufacturer’s directions. Four sequencing libraries were pooled per flow cell and sequenced on four GridION flow cells (Oxford Nanopore Technologies; R9.4.1) for 72 hours on a GridION Mk1 device (Oxford Nanopore Technologies; Oxford, England, UK). Raw data was live basecalled using the high accuracy model in Guppy (v.4.0.11).
Goike J., Hsieh C.L., Horton A., Gardner E.C., Bartzoka F., Wang N., Javanmardi K., Herbert A., Abbassi S., Renberg R., Johanson M.J., Cardona J.A., Segall-Shapiro T., Zhou L., Nissly R.H., Gontu A., Byrom M., Maranhao A.C., Battenhouse A.M., Gejji V., Soto-Sierra L., Foster E.R., Woodard S.L., Nikolov Z.L., Lavinder J., Voss W.N., Annapareddy A., Ippolito G.C., Ellington A.D., Marcotte E.M., Finkelstein I.J., Hughes R.A., Musser J.M., Kuchipudi S.V., Kapur V., Georgiou G., Dye J.M., Boutz D.R., McLellan J.S, & Gollihar J.D. (2021). Synthetic repertoires derived from convalescent COVID-19 patients enable discovery of SARS-CoV-2 neutralizing antibodies and a novel quaternary binding modality. bioRxiv.
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