α cd19

Poly (D, L-lactic-co-glycolic acid) (75:25 PLGA, Resomer® RG 752H, M_W 4A) was purchased from Lakeshore Biomaterials (Birmingham, AL, USA). Ovalbumin (OVA_257-264) and dimethyl-dioctadecyl-ammonium bromide (DDAB) were obtained from Sigma (St, Louis, MO, USA). Methylene chloride (AR grade) and absolute ethyl alcohol (AR grade) were purchased from Beijing Chemical Reagent Company (Beijing, China). Concanavalin A, Roswell Park Memorial Institute (RPMI) 1640 medium, Dulbecco's modified Eagle medium (DMEM) medium, and fetal bovine serum (FBS) were supplied by Gibco (Grand Island, NY, USA). Alexa 635-phalloidin and Lysol-Tracker probes were purchased from Invitrogen (Grand Island, NY, USA). FITC was obtained from Sigma-Aldrich. Fluorochrome-labeled α-MHCI, α-MHC II, α-CD86, α-CD80, α-CD11c, α-CD69, α-CD44, α-CD62L, α-CD4, α-CD8 and α-CD19 antibodies were supplied by eBioscience (San Diego, CA, USA). Recombinant mouse GM-CSF and IL-4 were obtained from Peprotech (Rocky Hill, NJ, USA). Mouse cytokine ELISA kits were purchased from eBioscience. OVA derived (H-2 Kb, SIINFEKL) specific MHC I pentamers was purchased from ProImmune (Oxford, UK). p38 MAPK inhibitor SB203580 was purchased from Cell Signaling Technology (Danvers, MA, USA).

Flow-cytometric analysis of single-cell suspensions from spleen, lymph nodes (inguinal, axillary, cervical and mesenteric lymph nodes), thymus, whole blood, bone marrow, and TILs was performed using α-CD45, α-CD8, α-CD4, α-CD19, α-CD44, α-CD25, α-CD122, α-FoxP3, α-IFN-γ (all eBioscience), α-TNF-α, α-Ki67, α-CD3 (BioLegend), α-NK1.1 (BD Pharmingen). Stainings for intracellular FoxP3, Ki67, TNF-α, and IFN-γ were performed following the manufacturer’s instructions (eBioscience).