Rabbit anti prps6

For immunohistochemical analysis, glioma tissues were obtained from 10 patients (Grade 1: 3 cases, Grade 2: 2 cases, Grade 3: 2 cases, Grade 4: 3 cases) at Kumamoto University Hospital, Japan, after obtaining informed consent from the patients and in accordance with the guidelines of the Research Ethics Committee (Kumamoto University Hospital No. 1470). All tissue specimens were rapidly frozen after surgery and stored at −80 °C. Glioma patient tissues were fixed by formalin overnight, embedded in paraffin, and then cut into 3-μm-thick sections. The following antibodies were used to detect anti-gens: rabbit anti-pRPS6 (1:100; Abcam), rabbit anti-Nestin (1:200; Merck), and mouse anti-CD34 (1:100; Leica). Immunohistochemical reactions were visualized using a diaminobenzidine substrate system (Nichirei chuo-ku, Tokyo, Japan).

For double immunofluorescence histochemical studies, resected GBM tissue was harvested from patients at Kumamoto University Hospital, Japan (research Ethics Committee Kumamoto University Hospital No. 1470). After GBM, patient tissue was fixed in formalin overnight, embedded in paraffin, and then cut into 3-μm-thick sections. The following antibodies were used to detect anti-gens: rabbit anti-pRPS6 (1:100; Abcam), rabbit anti-Nestin (1:200; Merck), and mouse anti-CD34 (Leica 1:100). Reactions were visualized by using a 2nd antibody rabbit-alexa594 and mouse-alexa488 (Thermo Fisher Scientific, Waltham, MA, USA). Nucleo was visualized by DAPI (Thermo Fisher Scientific). Fluorescence images were captured using a BZ-X700 (KEYENCE, Osaka, Japan).