The HPLC system used in the present study was Agilent 1200 Series equipped with Quaternary Pump, Agilent 1200 Series Vacuum Degasser, Agilent 1200 Series Autosampler and Agilent 1200 Series Diode Array Detector Purchased from Agilent Technologies (Hewlett-Packard Strasse Waldbronn, Germany). Chromatographic separation of the compounds was performed on a C18 analytical column (Techsphere 5ODS, 25 cm × 4.6 mm ID; HPLC Technology, Macclesfield, Cheshire, UK). Data acquisition and processing were accomplished with LC Chemstation software (Agilent Technologies). Adwa pH meter (AD1020 pH/mV/ISE/Temperature, Hungary) was used for the determination of the sample pH and A 800 model centrifuge, China, was used to speed up the phase separation. An electronic balance (Adam Equipment Company, UK) was utilized for weighing the different chemicals involved in the experiments. For the measurement of total dissolved solids (TDS) and electrical conductivity, conductivity meter (Postfach 24 80, Germany) was used.
Agilent 1200 series vacuum degasser
Manufactured by Agilent Technologies
Sourced in Germany, United States
The Agilent 1200 Series Vacuum Degasser is a laboratory equipment designed to remove dissolved gases from liquid samples. It operates by applying a vacuum to the liquid, which causes the gases to be extracted, resulting in a degassed sample.
Automatically generated - may contain errors
Lab products found in correlation
Agilent 1200 series diode array detector, by Agilent Technologies (1 mentions)
Agilent 1200 series autosampler, by Agilent Technologies (1 mentions)
Agilent 1200 series, by Agilent Technologies (1 mentions)
Symmetry c18 column, by Agilent Technologies (1 mentions)
Chemstation software, by Agilent Technologies (1 mentions)
2 protocols using agilent 1200 series vacuum degasser
1
HPLC Analysis of Compounds Using Agilent 1200 Series
2
Analytical Chromatographic Separation of Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The analytical chromatographic system including an Agilent 1200 series vacuum degasser, an automatic sample injector, a quaternary pump, a scanning fluorescence detector (all from Agilent Technologies, Palo Alto, CA, USA), The samples were separated on a Symmetry ® C18 column (3.5 μm particle diameter, 100 Å pore diameter, 4.6 mm id, 150 mm length, with matching guard column; Agilent Technologies, Palo Alto, USA) USA). The system was controlled controlled by a computer running Chem Station Software (Agilent Technologies). Analytical chromatography was carried out with a flow rate of 1.5 ml/min at 25°C ± 1°C. The gradient details are presented in [Table 1]. Excitation and emission wavelengths were 340 and 450 nm, respectively.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!