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Pcdna4 chk1 flag

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The pcDNA4-Chk1-Flag is a plasmid vector that expresses the Chk1 (Checkpoint kinase 1) protein with a Flag tag. Chk1 is a serine/threonine-protein kinase involved in the regulation of the cell cycle and the response to DNA damage.

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Lab products found in correlation

Dasatinib, by Selleck Chemicals (1 mentions) Etoposide, by Selleck Chemicals (1 mentions) Lats2, by Cell Signaling Technology (1 mentions) P chk1, by Cell Signaling Technology (1 mentions) P cdc25c, by Cell Signaling Technology (1 mentions) P chk2, by Cell Signaling Technology (1 mentions) P lat, by Cell Signaling Technology (1 mentions) Pd0325091, by Selleck Chemicals (1 mentions) P h2ax, by Cell Signaling Technology (1 mentions) Anti yap taz, by Cell Signaling Technology (1 mentions) Survivin, by Cell Signaling Technology (1 mentions) P mst1 2, by Cell Signaling Technology (1 mentions) Lats1, by Cell Signaling Technology (1 mentions) γh2ax, by Cell Signaling Technology (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) Pmek s217 221, by Cell Signaling Technology (1 mentions) Dna pk, by Cell Signaling Technology (1 mentions) Bibw2992, by Selleck Chemicals (1 mentions) P cdc2, by Cell Signaling Technology (1 mentions) P smad2, by Cell Signaling Technology (1 mentions)

2 protocols using pcdna4 chk1 flag

1

Investigating Signaling Pathways in Cancer

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Dasatinib, BIBW2992, PD0325091 and Etoposide were purchased from Selleck Chemicals (Houston, TX) and prepared as 20 mM stock solutions in DMSO. Antibodies used included anti-YAP-TAZ, pLats, Lats1, Lats2, pMST1/2, pH2AX, MEK, pMEK(S217/221), ERK, pERK(T202/Y204), pRb, pS6, LC3A/B, ATM, pChk1, Chk1, pChk2, Chk2, pCDC25c, pATRIP, p27, p21, pp38, β-catenin, cyclin D1, survivin, p4EBP1, pSMAD2(S465–467), p70S6K, pCDC2, CDC6, γH2AX, ATR, pDNA-PK(S2056) and DNA-PK, (Cell Signaling Technology, Danvers, MA); BRAF and cyclin E (Santa Cruz, Dallas, TX) and ATM, Flag M2 and β-actin (Sigma-Aldrich, St. Louis, MO); pATM(S1981) (GeneTex, Irvine, CA). Predesigned siRNAs of the target genes were purchased from Dharmacon (Pittsburgh, PA) or Thermo Scientific (Rodckford, IL). pcDNA4-Chk1-Flag and pCMV5-TOPO-3Xflag-TAZ plasmids were purchased from Addgene (Cambridge, MA). WTBRAF plasmid was provided by Dr. W. Kolch (Systems Biology Irland and The Conway Institute, University College Dublin).
Peng S., Sen B., Mazumdar T., Byers L.A., Diao L., Wang J., Tong P., Giri U., Heymach J.V., Kadara H.N, & Johnson F.M. (2015). Dasatinib induces DNA damage and activates DNA repair pathways leading to senescence in non-small cell lung cancer cell lines with kinase-inactivating BRAF mutations. Oncotarget, 7(1), 565-579.
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2

Plasmid Constructs for DNA Repair Studies

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pDEST pcDNA5-FRT/TO-eGFP-RIF1 was a gift from Daniel Durocher, The Lunenfeld-Tanenbaum Research Institute (Addgene plasmid no. 52506) (49 (link)). pcDNA4-CHK1-Flag was a gift from Aziz Sancar, University of North Carolina (Addgene plasmid no. 22894) (50 (link)). mCerulean-PCNA-19-SV40NLS-4 was a gift from Michael Davidson, Florida State University (Addgene plasmid no. 55386).
Moiseeva T.N., Yin Y., Calderon M.J., Qian C., Schamus-Haynes S., Sugitani N., Osmanbeyoglu H.U., Rothenberg E., Watkins S.C, & Bakkenist C.J. (2019). An ATR and CHK1 kinase signaling mechanism that limits origin firing during unperturbed DNA replication. Proceedings of the National Academy of Sciences of the United States of America, 116(27), 13374-13383.
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