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Cfi75 apochromat 25xc w

Manufactured by Nikon
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The CFI75 Apochromat 25XC W is a high-performance microscope objective lens manufactured by Nikon. It is designed for use in advanced laboratory applications. The lens features an apochromatic correction, which helps to minimize chromatic aberrations and provide sharp, high-contrast images across a wide range of wavelengths.

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Cfi75 lwd 16x w, by Nikon (2 mentions)

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CFI75 (9 citations)

2 protocols using cfi75 apochromat 25xc w

1

Two-Photon Imaging of Neural Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Two-photon (2P) imaging was performed on a Thorlabs Bergamo resonant scanning microscope with 920 nm excitation via a titanium:sapphire femtosecond laser (Chameleon Vision II, Coherent). A 1.1-NA 25× objective lens was used (CFI75 Apochromat 25XC W, Nikon Instruments) except for patching, where a 0.8-NA long-working distance 16× lens (CFI75 LWD 16X W, Nikon Instruments) was used to allow space for the patch pipette to approach the tissue under the microscope. The emission was split by a dichroic mirror into two channels: the green channel used a 525/50 nm filter, and the red channel used a 625/90 nm filter, before being collected by two photomultiplier tubes. ScanImage software (Vidrio) was used to control the microscope and acquire imaging data. Imaging power was kept between 20–70 mW depending on depth and field of view.
Liu Z., Lu X., Villette V., Gou Y., Colbert K.L., Lai S., Guan S., Land M.A., Lee J., Assefa T., Zollinger D.R., Korympidou M.M., Vlasits A.L., Pang M.M., Su S., Cai C., Froudarakis E., Zhou N., Patel S.S., Smith C.L., Ayon A., Bizouard P., Bradley J., Franke K., Clandinin T.R., Giovannucci A., Tolias A.S., Reimer J., Dieudonné S, & St-Pierre F. (2022). Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy. Cell, 185(18), 3408-3425.e29.
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2

Two-Photon Imaging of Neural Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Two-photon (2P) imaging was performed on a Thorlabs Bergamo resonant scanning microscope with 920 nm excitation via a titanium:sapphire femtosecond laser (Chameleon Vision II, Coherent). A 1.1-NA 25× objective lens was used (CFI75 Apochromat 25XC W, Nikon Instruments) except for patching, where a 0.8-NA long-working distance 16× lens (CFI75 LWD 16X W, Nikon Instruments) was used to allow space for the patch pipette to approach the tissue under the microscope. The emission was split by a dichroic mirror into two channels: the green channel used a 525/50 nm filter, and the red channel used a 625/90 nm filter, before being collected by two photomultiplier tubes. ScanImage software (Vidrio) was used to control the microscope and acquire imaging data. Imaging power was kept between 20–70 mW depending on depth and field of view.
Liu Z., Lu X., Villette V., Gou Y., Colbert K.L., Lai S., Guan S., Land M.A., Lee J., Assefa T., Zollinger D.R., Korympidou M.M., Vlasits A.L., Pang M.M., Su S., Cai C., Froudarakis E., Zhou N., Patel S.S., Smith C.L., Ayon A., Bizouard P., Bradley J., Franke K., Clandinin T.R., Giovannucci A., Tolias A.S., Reimer J., Dieudonné S, & St-Pierre F. (2022). Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy. Cell, 185(18), 3408-3425.e29.
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