Nc sirna

Manufactured by Tsingke

Sourced in China

NC) siRNA is a laboratory reagent used in molecular biology experiments. It is a form of small interfering RNA (siRNA) that serves as a negative control, designed to have no known target in the tested biological system.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) P3000, by Thermo Fisher Scientific (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Prl tk, by Beyotime (1 mentions) Lipo8000, by Beyotime (1 mentions) Poly 1 c, by Merck Group (1 mentions) Anti flag tag mouse monoclonal antibody, by Beyotime (1 mentions) Small interfering rna sirna, by Tsingke (1 mentions) Pisre luc, by Beyotime (1 mentions) Anti gapdh, by Beyotime (1 mentions) Mimic nc, by GenePharma (1 mentions) Mir 221 3p mimic, by GenePharma (1 mentions) Rpmi 1640 medium, by BasalMedia (1 mentions) Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)

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SiRNAs (13 citations)

5 protocols using nc sirna

Transient Transfection of KEAP1 Mutants

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Expression plasmids containing wild type and C368, C434, and C613 mutant KEAP1 genes, and siRNA targeting at GSTP (GSTP-siRNA) gene and the negative control (NC-siRNA) were provided by Tsingke Biotech (Beijing, China). For transient transfection, cells were cultured to 60%–80% confluence and then transfected using Lipofectamine™ 3000 and P3000™ reagent (Invitrogen, L3000015, USA) according to the manufacturer's instructions.
GSTP siRNA sense (5′-3′): GGCAAGGAUGACUAUGUGATT, antisense (5′-3′): UCACAUAGUCAUCCUUGCCTT.

Sun X., Guo C., Huang C., Lv N., Chen H., Huang H., Zhao Y., Sun S., Zhao D., Tian J., Chen X, & Zhang Y. (2024). GSTP alleviates acute lung injury by S-glutathionylation of KEAP1 and subsequent activation of NRF2 pathway. Redox Biology, 71, 103116.

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CCNB1 siRNA Knockdown in WIT-49 Cells

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Table 3 shows the CCNB1 targeting siRNA and negative control (NC) siRNA sequences (TSINGKE, Beijing, China). The WIT-49 cells were plated onto 6-well or 12-well plates and divided into four groups: experimental group for CCNB1siRNA-1, CCNB1siRNA-2, CCNB1siRNA-3, and negative control group with NC siRNA. Lipofectamine™ RNAiMAX (Invitrogen, USA) was used for transient transfection according to the manufacturer’s instructions.

Table 3

CCNB1 siRNA sequences

siRNA	Forward primer Sequence (5`−3`)	Reverse primer Sequence (5`−3`)
CCNB1 siRNA−1	GCUGAAUUCUGCACUAGUUTT	AACUAGUGCAGAAUUCAGCTT
CCNB1 siRNA−2	GGUAAAUCAAGGACUUACATT	UGUAAGUCCUUGAUUUACCTT
CCNB1 siRNA−3	CUGACAACACUUAUACUAATT	UUAGUAUAAGUGUUGUCAGTT

* The siRNA NC was provided in the reagent

Xiang B., Chen M.L., Gao Z.Q., Mi T., Shi Q.L., Dong J.J., Tian X.M., Liu F, & Wei G.H. (2023). CCNB1 is a novel prognostic biomarker and promotes proliferation, migration and invasion in Wilms tumor. BMC Medical Genomics, 16, 189.

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Interferon-beta Promoter Activation Assay

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The canine promoter reporter plasmid pIFNβ-Luc was constructed according to a previous study [25 (link)]; a pISRE-Luc and pRL-TK were purchased from Beyotime (Shanghai, China) and Promega (USA), respectively. Poly(I:C) (Sigma, USA) at a final concentration of 2 g/mL was the control for promoter activation experiments. Lipo8000 (Beyotime, China) was used for plasmid transfection according to the manufacturer’s instructions. Anti-GAPDH, anti-Flag-tag mouse monoclonal antibody and anti-IRF1 rabbit monoclonal antibody (Beyotime, China) were used in IFA and western blot. Small interfering RNA (siRNA) (+) against CaIRF1 sequence, 5′-GCACCAGUGACCUCUACAGTT-3′, the siRNA (−) sequence, 5′-CUGUAGAGGUCACUGGUGCTT-3′, and the negative control (NC) siRNA were purchased from Tsingke Biotech (Beijing, China). The primers used in the study were synthesized by Sangon Biotech (Shanghai, China).

Hao X., Chen H., Li Y., Chen B., Liang W., Xiao X., Zhou P, & Li S. (2022). Molecular characterization and antiviral effects of canine interferon regulatory factor 1 (CaIRF1). BMC Veterinary Research, 18, 440.

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Glucose-Induced Cytokine Regulation

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HaCaT cells that purchased from Pricell (Wuhan, China) and HUVEC cells that purchased from Guandao Biological Engineering (Shanghai, China) were cultured with Roswell Park Memorial Institute (RPMI) 1640 medium (BasalMedia, Shanghai, China) containing 10% phosphate buffered saline (PBS) as well as 1% penicillin/streptomycin. After reaching 50–70% confluence, cells were put into 6-well plates, followed by exposure to 3 levels of glucose (5.5, 35  or 50 mmol/L). After 24 hours of glucose treatment, HaCaT cells were then transfected by 100 nM of miR-221-3p mimics (GenePharma, China), siTHBS1 (100 nM, Tsingke Biotechnology, China) (forward: GGAGUUCAGUACAGAAAUAdTdT; reverse: UAUUUCUGUACUGAACUCCdTdT), siRNA NC (Tsingke Biotechnology, China) via Lipofectamine 3000 (L3000015, Thermo Fisher Scientific, USA) or mimic NC (100 nM, GenePharma, China) complying with the protocol provided by the manufacturer. Cells supernatant was collected to measure cytokine content while cells were harvested, preparing for subsequent analysis.

Hu K., Liu X., Chang H., Zhang Y., Zhou H., Liu L., Zhang X., Jiao Z., Shen B, & Zhang Q. (2023). MicroRNA-221-3p Targets THBS1 to Promote Wound Healing in Diabetes. Diabetes, Metabolic Syndrome and Obesity, 16, 2765-2777.

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Targeted Knockdown of ADAMTS5 and Ago2

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Small interfering RNA (siRNA) targeting human ADAMTS5 (siRNA-ADAMTS5) and Ago2 (siRNA-Ago2), and their scrambled control siRNAs (siRNA-NC), were purchased from TSINGKE (Nanjing, China). Cells were transfected using Lipofectamine 2000 reagent (Invitrogen) following the manufacturer’s instructions.

Duan A., Shen K., Li B., Li C., Zhou H., Kong R., Shao Y., Qin J., Yuan T., Ji J., Guo W., Wang X., Xue T., Li L., Huang X., Sun Y., Cai Z., Liu W, & Liu F. (2021). Extracellular vesicles derived from LPS-preconditioned human synovial mesenchymal stem cells inhibit extracellular matrix degradation and prevent osteoarthritis of the knee in a mouse model. Stem Cell Research & Therapy, 12, 427.

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