To achieve conditional deletion of SMAD4 specifically in CM, SMAD4fl/fl mice (Stock No. 017462, The Jackson Laboratory, Bar Harbor, Maine) were crossed with mice carrying the MerCreMer (MCM) transgene driven by the alpha-myosin heavy chain promoter (Myh6-Cre+/+) (Stock No. 005657, The Jackson Laboratory). Further details of generation and characterization of the CM-specific SMAD4 knockout (KO) mice are described in the Results section. The Institutional Animal Care and Use Committee of Vanderbilt University Medical Center approved all animal procedures and treatments used in this study (protocol #M1700133-00).
Smad4fl fl mice
Manufactured by Jackson ImmunoResearch
SMAD4fl/fl mice are a genetically engineered mouse strain that has the SMAD4 gene flanked by loxP sites, allowing for conditional deletion of the gene in specific cell types or tissues. SMAD4 is a key mediator in the transforming growth factor-beta (TGF-β) signaling pathway, which regulates various cellular processes. These mice provide a tool for studying the role of SMAD4 in different biological systems.
Automatically generated - may contain errors
2 protocols using smad4fl fl mice
1
Generation of Cardiomyocyte-Specific SMAD4 Knockout Mice
2
Conditional Gene Knockout in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Csf1rCre mice (#021024) and Smad4fl/fl mice (#017462) were purchased from Jackson Laboratory. The Smad2fl/fl mice were kind gifts from Dr. Daniel Bernard at McGill University. Human LangerinCre mice (9 (link)) were reported previously. All mice were backcrossed to C57BL/6J genetic background for at least six generations. The experiments were conducted in 7- to 12-week-old mice (both sexes) unless otherwise indicated. The mice were bred and maintained in a pathogen-free facility at the Laboratory Animal Services Center of Henry Ford Health System. Handling of mice and experimental protocols were in accordance with the Institutional Animal Care and Use Committee of Henry Ford Health System.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!