Bb fbs

The strains, plasmids and their relevant characteristics are shown in Supplementary Table S3 of the supporting information. E. coli strain was cultured using Luria-Bertini (LB, Lennox) broth (10 g tryptone, 5 g Yeast extract, 5 g NaCl per 1L). Solid medium (LB-agar) was prepared by addition of 1.5% agar [Nakalai tesque, Kyoto Japan]. If necessary, kanamycin (Km) and chloramphenicol (Cm) [FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan] was added to LB or LB-agar at 50 and 25 μg/ml, respectively. Brucella Broth (BB) [BD Bioscience, San Jose, CA, United States] supplemented with 10% fetal bovine serum (BB-FBS) [Cell Culture Laboratories, Cincinnati, OH, United States] was used for liquid culture of H. pylori. The solid media used were BB-FBS agar (BB-FBS, 1.5% agar), BB-HB agar (BB supplemented with 5% horse blood, 1.5% agar), or BD BBL Trypticase Soy Agar with 5% Sheep Blood (TSA) [BD Bioscience]. If necessary, Km and Cm were added to BB-FBS agar at 15 and 5 μg/ml, respectively. Both liquid cultures and agar plates were incubated at 37°C in the presence of 10% CO₂ and 5% O₂.