Anti-Src (cat. no. 2109), anti-phospho (p)-Akt (cat. no. 9271S), anti-p-p44/42 MAPK (Erk1/2) (cat. no. 9101) and anti-β-actin (cat. no. 4967) antibodies for western blotting were all purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Anti-p-Src Y419 (cat. no. AF2685) was purchased from R&D Systems, Inc. (Minneapolis, MN, USA). Dasatinib (Focus Biomolecules, Plymouth Meeting, PA, USA) was prepared as a 10-mmol/l stock solution in dimethyl sulfoxide (DMSO). Paclitaxel was purchased from Bristol-Myers Squibb (New York, NY, USA) and oxaliplatin was purchased from AdooQ Bioscience (Irvine, CA, USA).
Dasatinib
Manufactured by Focus Biomolecules
Sourced in United States
Dasatinib is a small-molecule inhibitor that blocks the activity of several tyrosine kinases, including BCR-ABL, SRC, c-KIT, PDGFR, and EPH receptor kinases. It is commonly used in biochemical and cell-based research applications.
Automatically generated - may contain errors
Lab products found in correlation
Oxaliplatin, by Adooq (1 mentions)
Paclitaxel, by Bristol-Myers Squibb (1 mentions)
Phospho akt, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Paclitaxel, by Fujifilm (1 mentions)
Cisplatin, by Fujifilm (1 mentions)
Etoposide, by Fujifilm (1 mentions)
Saracatinib, by Selleck Chemicals (1 mentions)
Docetaxel, by Fujifilm (1 mentions)
Cilengitide, by MedChemExpress (1 mentions)
Anti flag m2 antibody, by Merck Group (1 mentions)
4 protocols using dasatinib
1
Western Blotting Analysis of Src, Akt, and MAPK
2
Molecular Mechanisms of Dasatinib Action
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PJ2 was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Dasatinib was obtained from Focus Biomolecules (Plymouth Meeting, PA, USA) and dissolved in DMSO with a final concentration of 0.05% in the culture medium. MEK and phospho-MEK (ser217/221) antibodies were purchased from Santa Cruz Biotechnology, Inc., and SRC, phospho-SRC (tyr416), ERK, phospho-ERK (tyr204), AKT, phospho-AKT and β-actin antibodies were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).
3
Combinatorial Chemotherapeutic Agents
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VRB, cisplatin, paclitaxel, docetaxel, and etoposide were purchased from Wako Pure Chemical Industries (Osaka, Japan). The SFK inhibitor, dasatinib, was purchased from Focus Biomolecules (Plymouth Meeting, PA), and saracatinib was purchased from Selleck Chemicals (Houston, TX). The ABCB1 inhibitor, tariquidar, was purchased from Toronto Research Chemicals Inc. (Toronto, ON, Canada). Cilengitide (integrin αvβ3 inhibitor) was purchased from MedchemExpress (Monmouth Junction, NJ).
4
Integrin-Mediated Focal Adhesion Signaling
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Recombinant mouse and human OPN was purchased from Peprotech (Rocky Hill, NJ). Recombinant mouse HGF was provided by Mitsubishi Tanabe Pharma (Osaka, Japan). Recombinant mouse PDGF-AA was purchased from Prospec (Rehovot, Israel). Dasatinib, a specific FAK Y576/577 inhibitor, was purchased from Focus Biomolecules (Plymouth Meeting, PA). Cyclo αVβ3-integrin binding cyclic RGD peptide was from Anaspec (Fremont, CA). Anti-mouse CD29 neutralizing antibody was from Biolegend (San Diego, CA). Anti-mouse CD44 neutralizing antibody was from BD Biosciences (San Jose, CA). Antibodies recognizing total and phosphorylated (Y575/576) forms of FAK were obtained from Cell Signaling Technology (Danvers, MA). Antibodies against OPN and β-actin were from Santa Cruz Biotechnology (Santa Cruz, CA). M2 anti-Flag antibody was from Sigma-Aldrich (St. Louis, MO). The antibody against LMW-PTP was purchased from R&D systems (Minneapolis, MN).
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