Whole cell lysates from primary neurons and AFSC-EV were processed as previously described [25 (link)]. Primary antibodies were raised against the following molecules: Actin, Nox4 (Sigma-Aldrich, St Louis, MO, USA), Akt, SOD1, SIRT1, gp91phox, TrxR1, TrxR2, Gpx1, LC3β, PARP, TIA-1 (Santa Cruz Biotechnology, CA, USA), pAktser473 (Cell Signaling Technology, MA, USA), β-Amyloid clone 6E10 (Bio Legend, CA, USA), pTauser422 (OriGene Technologies, MD, USA), Rab5 (Lonza, SC, USA), CD9 (Life Technologies, CA, USA), CD81 (Thermo Fisher Scientific, MA, USA), Bcl-2 (Bio Source, CA, USA).
Secondary antibodies, used at 1 : 3000 dilution, were all from Thermo Fisher Scientific (Waltham, MA, USA).
Secondary antibodies, used at 1 : 3000 dilution, were all from Thermo Fisher Scientific (Waltham, MA, USA).