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Xcyto 2 sample slides

Manufactured by ChemoMetec
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The Xcyto 2-sample slides are a laboratory equipment product designed for cell counting and analysis. The slides provide a platform for preparing and analyzing two separate samples simultaneously. The core function of the Xcyto 2-sample slides is to enable efficient and accurate cell enumeration and characterization in a dual-sample format.

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Lab products found in correlation

Glass coverslip, by Corning (2 mentions) Bluemask 1, by ChemoMetec (2 mentions) Cellinsight cx7, by Thermo Fisher Scientific (2 mentions)

2 protocols using xcyto 2 sample slides

1

Live-Cell Imaging and Intracellular Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
1 × 105 cells adhered to glass coverslips (Corning), treated with MED2-CY7 were and imaged with EVOS FL (AMG) at 10X magnification for live cell imaging. MED2-CY7 localization accomplished in 4% Paraformaldehyde-fixed, 0.1% Triton-X permeabilized cells co-stained with 1:1000 BlueMask-1(ChemoMetec) and DAPI (2μg/mL), mounted on Xcyto 2-sample slides (ChemoMetec) and imaged with Xcyto10 at 20X magnification for intracellular quantification. Mean fluorescent intensity of nuclear and cytoplasmic compartments determined using XcytoView. CellInsight CX7 (ThermoFisher) SYTOX intensity quantification acquired using a heated chamber with CO2 and imaged in 5-minute intervals at 20X magnification using Hoechst and SYTOX orange channels.
Eustace N.J., Anderson J.C., Warram J.M., Widden H.N., Pedersen R.T., Alrefai H., Patel Z., Hicks P.H., Placzek W.J., Gillespie G.Y., Hjelmeland A.B, & Willey C.D. (2020). A cell-penetrating MARCKS mimetic selectively triggers cytolytic death in glioblastoma. Oncogene, 39(46), 6961-6974.
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2

Live-Cell Imaging and Intracellular Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
1 × 105 cells adhered to glass coverslips (Corning), treated with MED2-CY7 were and imaged with EVOS FL (AMG) at 10X magnification for live cell imaging. MED2-CY7 localization accomplished in 4% Paraformaldehyde-fixed, 0.1% Triton-X permeabilized cells co-stained with 1:1000 BlueMask-1(ChemoMetec) and DAPI (2μg/mL), mounted on Xcyto 2-sample slides (ChemoMetec) and imaged with Xcyto10 at 20X magnification for intracellular quantification. Mean fluorescent intensity of nuclear and cytoplasmic compartments determined using XcytoView. CellInsight CX7 (ThermoFisher) SYTOX intensity quantification acquired using a heated chamber with CO2 and imaged in 5-minute intervals at 20X magnification using Hoechst and SYTOX orange channels.
Eustace N.J., Anderson J.C., Warram J.M., Widden H.N., Pedersen R.T., Alrefai H., Patel Z., Hicks P.H., Placzek W.J., Gillespie G.Y., Hjelmeland A.B, & Willey C.D. (2020). A cell-penetrating MARCKS mimetic selectively triggers cytolytic death in glioblastoma. Oncogene, 39(46), 6961-6974.
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