Small interference RNA against circ_0005962 and negative control were synthesized by Sangon Biotech (Shanghai, China). The mimics of miR-382-5p (miR-382-5p), the inhibitor of miR-382-5p (anti-miR-382-5p), and respective negative control (NC or anti-NC) were purchased from Ribobio (Shanghai, China). The overexpression of circ_0005962 (circ_0005962) was performed as the previous study described [24 (link)] and constructed by Genechem (Shanghai, China), while the specific plasmid without the circ_0005962 cDNA was served as a control (circ-NC). The vector pcDNA3.1-PDK4 for the overexpression of PDK4 and the control pcDNA empty vector (vector) were constructed by Sangon Biotech. Lentiviral vector (Lenti-short hairpin) for stable NEAT1 downregulation (Lv-sh-circ_0005962) and the corresponding negative control (Lv-sh-NC) were obtained from Genechem. Cell transfection was conducted using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). Transfection efficiency and the following experiments were implemented after 48 h of transfection.
Anti mir 382 5p
Manufactured by RiboBio
Sourced in China
Anti-miR-382-5p is a lab equipment product designed to target and inhibit the expression of miR-382-5p, a specific microRNA. This product can be used in research applications to study the biological functions and regulatory roles of miR-382-5p.
Automatically generated - may contain errors
2 protocols using anti mir 382 5p
1
Modulating circular RNA in cells
2
Silencing circ_0005962 via RNA Interference
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Small interference RNA against circ_0005962 and negative control were synthesized by Sangon Biotech (Shanghai, China). The mimics of miR-382-5p (miR-382-5p), the inhibitor of miR-382-5p (anti-miR-382-5p) and respective negative control (NC or anti-NC) were purchased from Ribobio (Shanghai, China). The overexpression of circ_0005962 (circ_0005962) was performed as the previous study described (Y. Wang, J. Zhang, et al., 2019) and constructed by Genechem (Shanghai, China), while the specific plasmid without the circ_0005962 cDNA was served as control (circ-NC).
The vector pcDNA3.1-PDK4 (PDK4) for the overexpression of PDK4 and the control pcDNA empty vector (vector) were constructed by Sangon Biotech. Lentiviral vector (Lenti-short hairpin) for stable NEAT1 downregulation (Lv-sh-circ_0005962) and corresponding negative control (Lv-sh-NC) were obtained from Genechem. Cell transfection was conducted by using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). Transfection efficiency and following experiments were implemented after 48h of transfection.
The vector pcDNA3.1-PDK4 (PDK4) for the overexpression of PDK4 and the control pcDNA empty vector (vector) were constructed by Sangon Biotech. Lentiviral vector (Lenti-short hairpin) for stable NEAT1 downregulation (Lv-sh-circ_0005962) and corresponding negative control (Lv-sh-NC) were obtained from Genechem. Cell transfection was conducted by using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). Transfection efficiency and following experiments were implemented after 48h of transfection.
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