Cells were plated on coverslips overnight, fixed with 4% paraformaldehyde for 10 min, permeabilized with 0.2% Triton X-100 for 10 min, and incubated for one hour at room temperature with primary antibodies listed in Table 7, in 1X PBS 4% BSA. After primary antibody incubation, cells were washed in 1X PBS and incubated with secondary antibodies in PBS 4% BSA for 30 min at room temperature, and then washed in 1X PBS three times with a final wash in distilled water. Coverslips were mounted on slides using Invitrogen™ Fluoromount-G™ polymerizing medium.
Fluoromount g polymerizing medium
Manufactured by Thermo Fisher Scientific
Fluoromount-G™ is a polymerizing mounting medium designed for use in fluorescent microscopy. It is a water-based, non-crystallizing solution that helps preserve the fluorescence of labeled specimens.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fluoromount g polymerizing medium
1
Immunofluorescence Staining of Cultured Cells
2
Immunofluorescence Staining of Cultured Cells
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Cells were plated on coverslips overnight, fixed with 4% paraformaldehyde for 10 min, permeabilized with 0.2% Triton X-100 for 10 min, and incubated for one hour at room temperature with primary antibodies listed in Table 7, in 1X PBS 4% BSA. After primary antibody incubation, cells were washed in 1X PBS and incubated with secondary antibodies in PBS 4% BSA for 30 min at room temperature, and then washed in 1X PBS three times with a final wash in distilled water. Coverslips were mounted on slides using Invitrogen™ Fluoromount-G™ polymerizing medium.
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