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Phosphatase inhibitor cocktail 1 and 11

Manufactured by Merck Group
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Phosphatase inhibitor cocktails 1 and 11 are laboratory reagents designed to inhibit the activity of phosphatases, a class of enzymes that remove phosphate groups from proteins and other biomolecules. These cocktails are commonly used in protein extraction and purification procedures to preserve the phosphorylation status of proteins, which is crucial for understanding their regulatory mechanisms and signaling pathways.

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Lab products found in correlation

Protease inhibitor cocktail 111, by Merck Group (2 mentions)

Close spelling variants of this product

Phosphatase inhibitor cocktail Phosphatase I inhibitor Cocktail inhibitor Phosphatases inhibitors Phosphatase inhibitors Phosphatases

2 protocols using phosphatase inhibitor cocktail 1 and 11

1

PPM1G Phosphorylation Assay in Glioma Cells

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Glioma cells were pretreated, as indicated, with vehicle, LY294002, or MK-2206 for 1 hour followed by incubation in phosphate-free media containing vehicle/inhibitor(s) for 30 minutes. 200μCi 32P-orthophosphate/ml was then added for 2 hours. After labeling, cells were harvested/lysed in buffer containing 50mM Tris-Cl (pH 7.4), 150mM NaCl, 1mM EDTA, 1% Triton X-100, protease inhibitor cocktail 111 (Sigma), and phosphatase inhibitor cocktail 1 and 11 (Sigma). Immunoprecipitation with anti-PPM1G or anti-FLAG antibody was then performed, resolved by SDS-PAGE and electrotransferred to PVDF membrane. 32P-labeling of PPM1G was detected by phosphorimaging as described above. Immunoblotting was then performed using antibodies against PPM1G by standard procedures.
Xu K., Wang L., Feng W., Feng Y, & Shu H.K. (2016). Phosphatidylinositol-3 kinase-dependent translational regulation of Id1 involves the PPM1G phosphatase. Oncogene, 35(44), 5807-5816.
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2

PPM1G Phosphorylation Assay in Glioma Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Glioma cells were pretreated, as indicated, with vehicle, LY294002, or MK-2206 for 1 hour followed by incubation in phosphate-free media containing vehicle/inhibitor(s) for 30 minutes. 200μCi 32P-orthophosphate/ml was then added for 2 hours. After labeling, cells were harvested/lysed in buffer containing 50mM Tris-Cl (pH 7.4), 150mM NaCl, 1mM EDTA, 1% Triton X-100, protease inhibitor cocktail 111 (Sigma), and phosphatase inhibitor cocktail 1 and 11 (Sigma). Immunoprecipitation with anti-PPM1G or anti-FLAG antibody was then performed, resolved by SDS-PAGE and electrotransferred to PVDF membrane. 32P-labeling of PPM1G was detected by phosphorimaging as described above. Immunoblotting was then performed using antibodies against PPM1G by standard procedures.
Xu K., Wang L., Feng W., Feng Y, & Shu H.K. (2016). Phosphatidylinositol-3 kinase-dependent translational regulation of Id1 involves the PPM1G phosphatase. Oncogene, 35(44), 5807-5816.
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