Striatum was homogenized in RIPA buffer containing protease and phosphatase inhibitors (1 mM sodium orthovanadate, 1 mM aprotinin, and 1% protease inhibitor cocktail) and centrifuged at 10000g for 10 minutes at 4°C. Supernatants were used for western blotting, as described in da Rosa-Junior.33 (link) The following primary antibodies were used: anti-phospho-JNK and anti-JNK were purchased from R&D Systems, Minnesota; anti-Bok, anti-Bcl-xL, anti-caspase-9, anti-caspase-3, anti-cleaved caspase-3, anti-phospho-p38, anti-p38, anti-phospho-ERK1/2, and anti-ERK1/2 from Cell Signaling, Massachusetts; anti-superoxide dismutase 1 (SOD1), anti-heme oxygenase-1 (HO-1), and anti-α-synuclein from Abcam, Cambridge, UK; anti-catalase (CAT) was from Merk Millipore, Massachusetts; anti-GSK-3β from Santa Cruz Biotechnology, Texas; anti-β-actin from Sigma-Aldrich, Missouri.
Anti bok
Manufactured by R&D Systems
Sourced in United States
Anti-Bok is a recombinant human protein that functions as a member of the BCL-2 family of proteins. It plays a role in the regulation of apoptosis, or programmed cell death.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin, by Merck Group (1 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Anti α synuclein, by Abcam (1 mentions)
Anti gsk3β, by Santa Cruz Biotechnology (1 mentions)
Anti phospho jnk, by R&D Systems (1 mentions)
Anti caspase 9, by Cell Signaling Technology (1 mentions)
Anti erk1 2, by Cell Signaling Technology (1 mentions)
Anti p38, by Cell Signaling Technology (1 mentions)
Anti jnk, by R&D Systems (1 mentions)
Anti phospho erk1 2, by Cell Signaling Technology (1 mentions)
Anti bcl xl, by Cell Signaling Technology (1 mentions)
Anti phospho p38, by Cell Signaling Technology (1 mentions)
Anti caspase 3, by Cell Signaling Technology (1 mentions)
Halt protease phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions)
T5168, by Merck Group (1 mentions)
Ab662, by Abcam (1 mentions)
Anti xiap, by Cell Signaling Technology (1 mentions)
Ab26414, by Abcam (1 mentions)
Ab51037, by Abcam (1 mentions)
Ab27568, by Abcam (1 mentions)
2 protocols using anti bok
1
Striatum Protein Analysis by Western Blot
2
Immunoblotting Analysis of Apoptosis and Stem Cell Markers
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Cells were lysed in freshly prepared RIPA buffer supplemented with 1x Halt Protease/Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific). Cleared lysates were resolved by SDS-PAGE, transferred to PVDF membrane and incubated with primary antibodies. The antibodies applied in the current study are as follows: anti-BCL-2 (M0887, DAKO), anti-BID (#2002, Cell Signaling Technology), anti-BAG3 (NBP2-27398, NOVUS), anti-BOK (AF6067, R&D System), anti-BAK (SC-7873, Santa Cruz), anti-BAD (610391, BD Bioscience), anti-CASP9 (#9502, Cell Signaling Technology), anti-CYCS (556433, BD Bioscience), anti-BIRC5 (SURVIVIN) (#2802, Cell Signaling Technology), anti-XIAP (#2042, Cell Signaling Technology), GAPDH (AF5718, R&D System), anti-SNAI2 (SLUG) (ab27568, Abcam), anti-SOX9 (ab26414, Abcam), anti-total CD44 (ab51037, Abcam), anti-integrin α2β3 (ab662, Abcam), anti-HIF1A (AF1935, R&D System), anti-HIF2A (AF2886, R&D System) and α-tubulin (T5168, Sigma).
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