1290 infinity high performance liquid chromatograph

The major apparatus and instruments used in the study are as follow: Agilent Technologies 1290 Infinity high-performance liquid chromatograph equipped with a quaternary pump, a autosampler, and a DAD detector (Agilent Technologies, USA), Electronic balance (METLER, MS-105DU), Milli-Q Ultra-pure water system (Milli-Q DIRECT8, USA), Electronic Thermometer (OMRON MC-347), Hemorheological Analyser (Beijing Gtmsteellex Science Instrument Co., Ltd), Fully Automatic Hemocyte Analyzer (Nihon Kohden Corporation, Japan, MEK~6318K), Platelet Aggregation and Coagulation Factor Analyzer (Beijing Steellex Scientific Instrument Company, LG-PABER), and centrifuge (ShangHai Anting Scientific Instrument Factory).

All samples and controls were analyzed via LCMS using an Agilent 1,290 Infinity high performance liquid chromatograph (HPLC) coupled to an Agilent 6,540 Ultra High Definition (UHD) quadrupole time-of-flight (Q-TOF) mass spectrometer. A detailed outline of the experimental conditions, instrument parameters, and injection order are provided in the Supplementary Material S3. Each set of triplicate preparations was measured using full scan mode (MS¹) with a single injection (20 μL) for each ionization mode. The final replicate for each triplicate set was injected an additional time (with a DDA method to collect MS² spectra, where charged monoisotopic masses ([M + H] for positive ion mode and [M-H] for negative ion mode). DDA was performed independently of the suspect screening list; after review of the MS-level data, potential detections from the suspect screen included as a preferred-ions list that were not selected for fragmentation via DDA were targeted for fragmentation in an additional injection.