Aβ25 35

Manufactured by ChinaPeptides

Aβ25−35 is a peptide fragment commonly used in laboratory research. It is a segment of the amyloid-beta protein, which is associated with Alzheimer's disease. This product is intended for use in scientific research and experiments, and its core function is to serve as a tool for studying amyloid-beta related processes.

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Lab products found in correlation

Mito tracker red cmxros, by Beyotime (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Solarbio (1 mentions) Donepezil hydrochloride, by Eisai (1 mentions)

2 protocols using aβ25 35

Amyloid-beta Peptide Toxicity Assay

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Aβ₂₅₋₃₅ was synthetized by the ChinaPeptides Co., Ltd. (Shanghai,
China). Dorsomorphin (Compound C, B3252) and G418 (A2513) were purchased from
APExBIO (Houston, USA). AMPK mouse monoclonal antibody (bsm-33236 M) and Sirt1
rabbit ployclonal antibody (bs-0921R) were purchased from Biosynthesis
Biotechnology Co., Ltd. (Beijing, China). PGC1α rabbit antibody (ab54481) was
obtained from Abcam (Shanghai, China). Caspase-3 rabbit monoclonal antibody
(D3R6Y) was obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). Aβ
mouse monoclonal antibody (TA500973S) was obtained from Origene (Rockville, MD,
USA). Active Oxygen Detection Kit (S0033S), Annexin V-mCherry Apoptosis
Detection Kit (C1070 M), Mito-Tracker Red CMXRos (C1035), and pCMV-Mito-AT1.03
(D2606) were obtained from Beyotime (Shanghai, China).
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) was purchased
from Solarbio (Beijing, China).

Jia J., Yin J., Zhang Y., Xu G., Wang M., Jiang H., Li L., Zeng X, & Zhu D. (2023). Thioredoxin-1 Promotes Mitochondrial Biogenesis Through Regulating AMPK/Sirt1/PGC1α Pathway in Alzheimer's Disease. ASN NEURO, 15, 17590914231159226.

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Donepezil and Aβ 25-35 Protocol

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AOF consists of a mixture of three types of bioactive compounds, including polysaccharides, volatile oils, and ferulic acid (table 2). Donepezil hydrochloride (Eisai China Inc., China) was dissolved in sterile distilled water at a concentration of 9 μg/μL. Aβ 25-35 (ChinaPeptides Co., Ltd., China) was dissolved in 0.9% sterile physiological saline at a concentration of 10 μg/μL and incubated at 37°C for 4 days to induce aggregation before usage.

Wang H.P., Wu H.Y., Ma C.L., Zeng Q.T., Zhu K.M., Cui S.M., Li H.L., Wu G.T., Wu Z.W, & He J.Z. (2022). Optimal Formula of Angelica sinensis Ameliorates Memory Deficits in β-amyloid Protein-induced Alzheimer's Disease Rat Model. Current medical science, 42(1).

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