Platelia aspergillus enzyme immunoassay eia

A Platelia Aspergillus enzyme immunoassay (EIA) (Bio-Rad, Marnes-La-Coquette, France) was used to assess the GM antigen indices of the sputum supernatants according to the manufacturer’s recommended protocol; an index of ≥1 was considered positive, and assays with positive results were repeated in duplicate (63 (link), 64 (link)).

The Platelia Aspergillus enzyme immunoassay (EIA) (Bio-Rad Laboratories, Redmond, WA) was performed according to the manufacturer’s procedures at MiraVista Laboratories (Indianapolis, IN, Ref [13 (link)]). Results were expressed as GM indices defined as the ratio of the optical density (OD) value of the sample to the OD value of a standard sample containing 1 ng of GM.
Independent testing of GM was performed on all IPA cases using serum collected on the same day as samples used for proteomics analysis. The median number of days between clinical diagnostic serum GM and independent testing was 3.5 days (range 41 days before clinical diagnostic to 57 days after clinical diagnostic). GM and independently tested serum GM were the same (positive or negative) in 44 of the 60 cases tested (S2 Table). Of the 14 cases where clinical GM and study GM differed, all clinical diagnostic GM tests were positive and independently tested GM were negative. In addition, of these 14 cases, 13 were undergoing antifungal therapy at the time of collection of the independent tested GM sample, and in 1 case, the sample was collected 4 days after antifungal therapy concluded. Only serum was used for GM analysis. The cut off for determination of positive GM was GM ≥ 0.5.