Fam83g

Manufactured by Abcam

FAM83G is a protein-coding gene that is involved in various cellular processes. It is expressed in multiple tissues and has been associated with various biological functions, but its precise role is not fully understood. This product provides a reagent for further research into the function and regulation of the FAM83G gene and its encoded protein.

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Lab products found in correlation

Irdye 800cw donkey anti goat igg, by LI COR (2 mentions) Irdye 800cw goat anti rat igg, by LI COR (2 mentions) Starbright blue 700 goat anti rabbit igg, by Bio-Rad (2 mentions) Starbright blue 700 goat anti mouse igg, by Bio-Rad (2 mentions) Lamin a c, by Cell Signaling Technology (2 mentions) α tubulin, by Thermo Fisher Scientific (2 mentions) Ubiquitin bml pw8810, by Enzo Life Sciences (1 mentions) Zfp91, by Fortis Life Sciences (1 mentions) Hif 1α, by BD (1 mentions) Mouse monoclonal anti ha, by Merck Group (1 mentions) Phospho β catenins33 s37 t41, by Cell Signaling Technology (1 mentions) Phospho β catenin s45, by Cell Signaling Technology (1 mentions) β catenin d10a8, by Cell Signaling Technology (1 mentions) Mouse anti gfp clone 7.1 and 13 1, by Roche (1 mentions) Irdye 680lt goat anti rabbit, by LI COR (1 mentions) Irdye 800cw goat anti mouse, by LI COR (1 mentions)

2 protocols using fam83g

Antibody Characterization for FAM83 and CK1

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Antibodies recognising FAM83B (SA270), FAM83D (SA102), FAM83F (SA103), FAM83H (SA273), CK1α (SA527), CK1ε (SA610), CK1δ (SA609), and GFP (S268B) were generated in-house and are available for request from the MRC-PPU reagents website (http://mrcppureagents.dundee.ac.uk). Antibodies recognising GAPDH (14C10) (#2118), IKZF1 (D6N9Y) (#14859), CRBN (D8H3S) (#71810), Na, K-ATPase alpha1 (D4Y7E) (#23565), and Lamin A/C (#2032) were obtained from Cell Signalling Technology. Additional antibodies used were FAM83G (ab121750; Abcam), α-tubulin (MA1-80189; Thermo Fisher Scientific), Ubiquitin (BML-PW8810; Enzo), HIF-1α (6109590; BD Biosciences), and ZFP91 (A303-245A; Bethyl Laboratories). Secondary antibodies used were StarBright Blue 700 goat anti-rabbit IgG (12004161; Bio-Rad), StarBright Blue 700 goat anti-mouse IgG (12004158; Bio-Rad), IRDye 800CW donkey anti-goat IgG (926-32214; LI-COR) and IRDye 800CW goat anti-rat IgG (926-32219; LI-COR).

Dunbar K., Macartney T.J, & Sapkota G.P. (2020). IMiDs induce FAM83F degradation via an interaction with CK1α to attenuate Wnt signalling. Life Science Alliance, 4(2), e202000804.

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Antibodies for Cellular and Molecular Analysis

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Antibodies recognising GFP (S268B), CK1α (SA527), CK1δ (SA609), CK1ε (SA610), and FAM83F (SA103) are available on request from the MRC-PPU reagents website (http://mrcppureagents.dundee.ac.uk). Antibodies for β-actin (#4967), GAPDH (14C10) (#2118), p-LRP6 (S1490) (#2568), LRP6 (C47E12) (#3395), β-catenin (D10A8) (#8480), phospho-β-catenin (S45) (#9564), phospho-β-catenin (S33/S37/T41) (#9561), Na/K ATPase (D4Y7E) (#23565), and Lamin A/C (#2032) were purchased from Cell Signalling Technology. Additional antibodies used were FAM83G (ab121750; Abcam), α-tubulin (MA1-80189; Thermo Fisher Scientific), Active-β-catenin (anti-ABC) clone 8E7 (05-665; END Millipore), mouse anti-GFP clone 7.1 and 13.1 (Roche), mouse-monoclonal anti-HA (H9658; Sigma-Aldrich), and U2AF1 (PA5-28510; Thermo Fisher Scientific). Secondary antibodies used were StarBright Blue 700 Goat anti-Rabbit IgG (12004161; Bio-Rad), StarBright Blue 700 Goat anti-Mouse IgG (12004158; Bio-Rad), IRDye 800CW Donkey anti-Goat IgG (926-32214; LI-COR), IRDye 800CW Goat anti-Rat IgG (926-32219; LI-COR), IRDye 800CW Goat anti-Mouse (926-32210; LI-COR), and IRDye 680LT Goat anti-Rabbit (926-68021; LI-COR).

Dunbar K., Jones R.A., Dingwell K., Macartney T.J., Smith J.C, & Sapkota G.P. (2020). FAM83F regulates canonical Wnt signalling through an interaction with CK1α. Life Science Alliance, 4(2), e202000805.

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