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Alexa flourtm 488 goat anti rabbit igg

Manufactured by Thermo Fisher Scientific
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Alexa Fluor™ 488 goat anti-rabbit IgG is a secondary antibody conjugate designed for use in immunofluorescence and other fluorescence-based applications. It is a goat-derived antibody specific to rabbit immunoglobulin G (IgG) that has been labeled with the Alexa Fluor® 488 fluorescent dye.

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Lab products found in correlation

Tcs sp8 laser confocal microscope, by Leica camera (1 mentions) Leica application suite x v2, by Leica camera (1 mentions) Antibody dilution buffer, by Beyotime (1 mentions)

2 protocols using alexa flourtm 488 goat anti rabbit igg

1

Immunofluorescence Microscopy Visualization

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Cells were grown in 4-well chamber slides, fixed with 4% paraformaldehyde, and incubated overnight with primary antibodies or Isotype Normal Rabbit IgG (R&D System, AB-105-C). Proteins were visualized by incubation with Alexa FlourTM 488 goat anti-rabbit IgG (Invitrogen, #A11034; Invitrogen). Nuclei were visualized by incubation with DAPI (1:5000). The coverslips or slides were covered with a mounting medium (Dako, #S302380). Fluorescence was monitored with a Leica TCS SP8 confocal laser microscope (Manheim, Germany) and processed using Leica Application Suite X v2.0.1.14392. The antibodies used are listed in Supplementary Table 6.
Zhang S., Yun D., Yang H., Eckstein M., Elbait G.D., Zhou Y., Lu Y., Yang H., Zhang J., Dörflein I., Britzen-Laurent N., Pfeffer S., Stemmler M.P., Dahl A., Mukhopadhyay D., Chang D., He H., Zeng S., Lan B., Frey B., Hampel C., Lentsch E., Gollavilli P.N., Büttner C., Ekici A.B., Biankin A., Schneider-Stock R., Ceppi P., Grützmann R, & Pilarsky C. (2024). Roflumilast inhibits tumor growth and migration in STK11/LKB1 deficient pancreatic cancer. Cell Death Discovery, 10, 124.
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2

Immunofluorescence Staining of GSDMD

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HCECs were cultured on eight-chamber slides and fixed with 4% paraformaldehyde in PBS for 15 min, then permeabilized with 0.5% Triton X-100 for 15 min, and blocked with 10% goat serum for 1 h. Samples were stained by anti-GSDMD antibody (1:50, Invitrogen) in antibody dilution buffer (Beyotime, Shanghai, China) at 4 °C overnight. Secondary staining was performed with Alexa FlourTM 488 goat anti-rabbit IgG (1:200, Invitrogen, USA) for 1 h at room temperature, and the nuclei were counterstained with DAPI for 7 min.
Li J., Yang K., Pan X., Peng H., Hou C., Xiao J, & Wang Q. (2022). Long Noncoding RNA MIAT Regulates Hyperosmotic Stress-Induced Corneal Epithelial Cell Injury via Inhibiting the Caspase-1-Dependent Pyroptosis and Apoptosis in Dry Eye Disease. Journal of Inflammation Research, 15, 3269-3283.
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