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2 protocols using sybr green rt pcr reaction kit

1

Benzyl Cinnamate Modulates Cellular Responses

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Foetal bovine serum (FBS), phosphate buffered saline (PBS), penicillin-streptomycin, trypsin-EDTA and Dulbecco’s modified Eagle medium (DMEM) were purchased from GIBCO (Grand Island, NY, USA). The human TNF-α (tumour necrosis factor-α) was purchased from Pepro Tech (Rocky Hill, NJ, USA). Griess reagent, dimethyl sulfoxide (DMSO), Cell Counting Kit-8 (CCK-8 kit), BCA Protein Assay Kit, and Annexin V-FITC/PI apoptosis kits were purchased from the BOSTER Biol.Tech. Co. (Wuhan, China). Cell Cycle Staining kits were purchased from Beyotime Biotechnology Company (Haimen, China). TRIzol™ Plus RNA Purification Kit (Invitrogen life technology, Carlsbad, CA, USA), ReverTra Ace® qPCR RT Master Mix (Beans Biol Tech. Co., Tokyo, Japan) and SYBR Green RT-PCR reaction kit (QPK-201, Toyobo, Tokyo, Japan) were used in RT-PCR experiment. Benzyl cinnamate was purchased from the Desite Biology Co., Ltd., (Chengdu, China).
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2

Cytotoxic and Apoptotic Effect Analysis

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Fetal bovine serum (FBS), phosphate buffered saline (PBS), Penicillin-Streptomycin, trypsin-EDTA and Dulbecco's modi ed Eagle medium (DMEM) were purchased from GIBCO (Grand Island, NY, USA). The human TNF-α was purchased from Pepro Tech (Rocky Hill, NJ, USA). Griess reagent, dimethyl sulfoxide (DMSO), Cell Counting Kit-8 (CCK-8 kit), BCA Protein Assay Kit, and Annexin V-FITC/PI apoptosis kits were purchased from the BOSTER Biol.Tech. Co. (Wuhan, China). Cell Cycle Staining kits were purchased from Beyotime Biotechnology Company (Haimen, China). TRIzol™ Plus RNA Puri cation Kit (Invitrogen life technology, Carlsbad, CA, USA), ReverTra Ace® qPCR RT Master Mix (Beans Biol Tech. Co., Tokyo, Japan) and SYBR Green RT-PCR reaction kit (QPK-201, Toyobo, Tokyo, Japan) were used in RT-PCR experiment.
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