Hc067047

Manufactured by MedChemExpress

Sourced in United States

HC067047 is a laboratory reagent. It is a small organic compound that can be used as a research tool in scientific experiments. The core function of HC067047 is to serve as an analytical standard or reference material for chemical analysis and characterization. No further details on the intended use or application of this product are available.

Automatically generated - may contain errors

Lab products found in correlation

Gsk1016790a, by MedChemExpress (3 mentions) Ouabain, by MedChemExpress (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Nifedipine, by Merck Group (1 mentions) Fura 2, by Thermo Fisher Scientific (1 mentions) Cyclopiazonic acid, by Merck Group (1 mentions) Gadolinium chloride, by Merck Group (1 mentions) Carbamylcholine chloride, by Merck Group (1 mentions) Skf 96365, by MedChemExpress (1 mentions) Gsk 7975a, by MedChemExpress (1 mentions) Dantrolene, by Apexbio (1 mentions) Fura 2 am, by Thermo Fisher Scientific (1 mentions) Dextran sulfate sodium (dss), by MP Biomedicals (1 mentions) Indomethacin, by Merck Group (1 mentions) Caffeine, by Merck Group (1 mentions) Carbachol, by Merck Group (1 mentions) Capsaicin, by MedChemExpress (1 mentions) Mpo assay kit, by Abcam (1 mentions) U73122, by Bio-Techne (1 mentions)

4 protocols using hc067047

Pharmacological Modulation of Calcium Signaling

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Prostaglandin E₂, Serotonin hydrochloride(5-HT), SKF-96365, TPEN (N, N, N′, N′- tetrakis (2-pyridylmethyl) ethylenediamine), GSK-7975A, ouabain, HC067047, and GSK1016790A were purchased from MedChemExpress (MCE; Monmouth Junction, NJ, United States). Sigma (Saint Louis, MO, United States) supplied carbamylcholine chloride (CCh), nifedipine, gadolinium chloride, and cyclopiazonic acid (CPA). Tocris Bioscience (Ellisville, MO, United States) supplied 2-Aminoethoxydiphenyl borate (2-APB), while APExBIO Technology LLC (Houston, TX, United States) provided dantrolene. Fura-2 was purchased from Invitrogen (UT, United States), meantime DMEM and FBS were obtained from Hyclone (Logan, UT, United States). Trypsin and penicillin/streptomycin were purchased from Gibco (CA, United States). The other chemicals were obtained from BBI Life Science (Shanghai, China).

Cui Y., Chu F., Yin K., Chen X., Wan H., Luo G., Dong H, & Xu F. (2021). Role of Serosal TRPV4-Constituted SOCE Mechanism in Secretagogues-Stimulated Intestinal Epithelial Anion Secretion. Frontiers in Pharmacology, 12, 684538.

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TRPV4-Mediated Inflammation Regulation

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All salts were supplied by Sangon Biotech. Glucose, caffeine, carbachol, and indomethacin were purchased from Sigma-Aldrich. Capsaicin, ouabain, SB705498, RN1734, RN1747, U73343, HC067047, GSK1016790A, methyl arachidonyl fluorophosphonate, AA, miconazole, and nystatin were purchased from MedChemExpress (MCE; Monmouth Junction). DSS was from MP Biomedicals (MP Bio). U73122 was from Tocris Bioscience (Tocris). Amiloride hydrochloride was from Molecular Probes, Inc (Molecular Probes). TNF-α and Fura-2/AM (Cat# F1221, Invitrogen) was from Invitrogen. TRPV4 (Abcam Cat# ab39260, RRID: AB_1143677), alpha 1 Sodium Potassium ATPase (phospho Y10) (Abcam Cat# ab124677, RRID: AB_10974461), Alexa Fluor 488 labeled anti-rabbit (Abcam Cat# ab150077, RRID: AB_2630356) antibodies, and MPO Assay Kit were from Abcam. Anti-GAPDH (Proteintech Cat# 60004-1-Ig, RRID: AB_2107436) was from Proteintech.

Wan H., Chen X.Y., Zhang F., Chen J., Chu F., Sellers Z.M., Xu F, & Dong H. (2022). Capsaicin inhibits intestinal Cl- secretion and promotes Na+ absorption by blocking TRPV4 channels in healthy and colitic mice. The Journal of Biological Chemistry, 298(5), 101847.

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Murine Trabecular Meshwork Explant Culture

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For explant culture, murine TMs were floated in Advanced DMEM supplemented with 1% penicillin-streptomycin. They were maintained in an incubator at 37°C with 5% CO₂. For drug exposures, the TMs were cultured in media with drug for 48 hours and then pulsed with 10 μM EdU for two hours. Whole-mounts were then processed as described above. Compounds tested in the screen are listed in Table S5 (Selleckchem). Crenolanib used after the initial screen was from MedChem Express, and CP-673451 used after the initial screen was from Tocris. We used additional small molecule inhibitors to treat TM explants and organoids in concentration response: BGJ398 (Cayman Chemical), PRN1371 (MedChem Express), HC-067047 (MedChem Express), GW788388 (Selleckchem), and SB-525334 (Cayman Chemical).

Frumm S.M., Yu K.S., Chang J., Artichoker J.A., Scaria S.M., Lee K.P., Byrnes L.E., Sneddon J.B, & Tward A.D. (2020). A hierarchy of proliferative and migratory keratinocytes maintains the tympanic membrane. Cell stem cell, 28(2), 315-330.e5.

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Chromenone Compound GL-V9 Protocol

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GL-V9 (C₂₄H₂₇NO₅, (5-hydroxy-8-methoxy-2-phenyl-7-(4-(pyrro-lidin-1-yl) butoxy)4H-chromen-4-one), MW 409.47, purity >99%) was kindly provided by Prof. Zhiyu Li (China Pharmaceutical University, China) and dissolved in dimethyl sulfoxide (DMSO, Sigma-Aldrich, St. Louis, MO, USA) to 0.01 M as the parent solution stored at −80 °C. TRPV4 specific agonist GSK1016790A (# HY-19608) and inhibitor HC067047 (# HY-100208) and the myosin II inhibitor blebbistatin (#HY-13441) were purchased from MedChem Express and dissolved in DMSO to 5 mM stored at −80 °C.

Liu J., Guo Y., Zhang R., Xu Y., Luo C., Wang R., Xu S, & Wei L. (2023). Inhibition of TRPV4 remodels single cell polarity and suppresses the metastasis of hepatocellular carcinoma. Cell Death & Disease, 14(6), 379.

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