5 ethynyl 2 deoxyuridine edu proliferation assay kit

Rabbit anti-human HOXA5 (Cat. #ab82645), p21 (Cat. #ab227443) and c-Myc (Cat. #ab32072) antibodies were purchased from Abcam (Cambridge, UK). Rabbit anti-human Ki67 (Cat. #AP19895b) antibody was purchased from Abgent (San Diego, CA, USA). Rabbit anti-human GAPDH (Cat. #10494-1-AP), cyclin D1 (Cat. #12363-1-AP) and cyclin E (Cat. #11554-1-AP) antibodies and horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibody (Cat. #SA00001-15) were purchased from Proteintech (Wuhan, China). Immunohistochemistry-related reagents, western blot analysis-related reagents, CCK-8 reagents and DAPI were purchased from Beyotime (Shanghai, China). All reagents used in RT-qPCR were purchased from Takara (Dalian, China). The 5-ethynyl-2′-deoxyuridine (EdU) proliferation assay kit was obtained from Ribobio (Guangzhou, China). All primers used for RT-qPCR were obtained from Sangon Biotech (Shanghai, China).

The Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). The 5-ethynyl-2-deoxyuridine (EdU) proliferation assay kit was purchased from Guangzhou RiboBio Co., Ltd. (Guangzhou, China). Anti-marker of proliferation Ki-67 (MKI67, 1:50 dilution, cat. no. 550609) and Matrigel were purchased from BD Biosciences (San Jose, CA, USA). The primary antibodies Wnt3a (cat. no. ab28472) were obtained from Abcam Company (Cambridge, MA, USA), the primary antibodies Wnt5a/b (cat. no. 2530), t-β-catenin (cat. no. 9562), p-β-catenin (cat. no. 2009), t-GSK-3β (cat. no. 12456), p-GSK-3β (cat. no. 9336), t-p38 (MAPK14, cat. no. 9212); p-MAPK14 (cat. no. 4511), β-actin (cat. no. 4967) and horseradish peroxidase (HRP)-conjugated secondary antibodies (cat. nos. 7074 and 7076) were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). All the chemicals used in the present study were of analytical grade (30 (link)).