The soil DNA extraction kit (QIAGEN Korea) was used for extracting DNA, according to the instructions of the manufacturer, the concentration of DNA was quantified using Nano-Drop (model One C), and the samples were preserved at −80°C for further proceedings.
Soil dna extraction kit
Manufactured by Qiagen
Sourced in United States
The Soil DNA extraction kit is a laboratory equipment product designed to extract and purify DNA from soil samples. It is a tool for researchers and scientists to obtain high-quality genomic DNA from various soil types for downstream molecular analysis applications.
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6 protocols using soil dna extraction kit
1
Soil DNA Extraction Using QIAGEN Kit
2
Soil DNA Extraction and Quantification
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DNA was extracted using the soil DNA extraction kit according to the manufacturer’s instruction (QIAGEN, Korea Ltd., Seoul, Korea), and the concentration of DNA in each sample was quantified using Nano drop (model one C). The extracted DNA samples were preserved at −80 °C for the follow-up experiments.
3
Microbial Profiling of Fruit Samples
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We collected five biological replicate samples for each fruit type: Each fly sample consisted of five female flies (wild and lab-reared flies) that emerged from a single fruit, and approximately 1g of fruit was excised with sterilized bladed for the corresponding fruit samples. DNA extractions were performed using Qiagen Soil DNA extraction kit according to manufacturer's protocol. Prior to extraction, pooled flies in 2mL centrifugation tubes were washed once in sterile water by vortexing for approximately 10 seconds to remove saw dust. Finally, in order to check for any kit-associated contamination (Glassing et al., 2016) , five extraction controls with no samples were subjected to DNA extraction and further sequencing.
Following extractions, we quantified DNA using NanoDrop2000 (Thermo Fisher Scientific, MA, USA). Sequencing was outsourced to the University of Minnesota Genomics Center (USA), on Illumina MiSeq platform (V3 Chemistry and 2x300 paired end run), targeting the V4-V6 region of the 16S rRNA gene and ITS1 region for bacteria and fungi respectively (Primer sequence information in supplementary file).
Following extractions, we quantified DNA using NanoDrop2000 (Thermo Fisher Scientific, MA, USA). Sequencing was outsourced to the University of Minnesota Genomics Center (USA), on Illumina MiSeq platform (V3 Chemistry and 2x300 paired end run), targeting the V4-V6 region of the 16S rRNA gene and ITS1 region for bacteria and fungi respectively (Primer sequence information in supplementary file).
4
Soil DNA Extraction Protocol
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DNA was extracted using the soil DNA extraction kit of MoBio Laboratories Inc (Carlsbad, CA, USA) according to manufacturer’s instructions. DNA was stored at −20 °C until required for molecular analysis.
5
Soil DNA Extraction Protocol
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For total soil DNA extraction, 0.5 g of soil sample was used for each soil type of red and paddy soils by following the instructions of the soil DNA extraction kit (MO-BIO Laboratories, Carlsbad, CA-USA). To check DNA purity and concentration, agarose gel electrophoresis and a Nanodrop-2000 spectrophotometer (Thermo-Scientific) were used. An adequate sample quantity was collected in a centrifuge tube, diluted with sterile water to 10 ng/μl, and stored at −40 °C for further analysis.
6
Soil DNA Extraction and Analysis
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Total soil DNA was extracted from the 0.5 g of a soil sample by Soil DNA-Extraction-Kit (MO-BIO Laboratories, Carlsbad, CA-USA) using the manufacturer's protocol. The agarose gel-electrophoresis and Nanodrop (Thermo Scientific Nanodrop 2000) were used to detect DNA purity and concentration. An appropriate amount of sample was taken in a centrifuge tube, diluted the sample with sterile water to 10 ng/µl, and stored in a refrigerator (-40ºC) for subsequent analyses.
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