Acid debranched wheat arabinoxylan

PASC was produced from Avicel PH-101 according to Wood et al. [64 ]. Avicel PH-101 (from cotton linters) was from Sigma-Aldrich, beechwood glucuronoxylan (product code BI3856) was from Apollo Scientific (Cheshire, UK), low viscosity wheat arabinoxylan (38:62 Ara:Xyl, product code P-WAXYL), enzymatically debranched wheat arabinoxylan (30:70 Ara:Xyl, product code P-EDWAX30) and acid debranched wheat arabinoxylan (22:78 Ara:Xyl, product code P-ADWAX22) were purchased from Megazyme (Bray, Ireland). All WAX substrates were reported to be > 94% pure on a dry weight basis (info for BeGX was not available). AGX from spruce was produced in-house as described in [40 (link)]. All substrates were dissolved to 1% (w/v) in water (the Megazyme substrates according to the producer’s manual), except for Avicel, which was suspended at 1% (w/w) in water.

Beechwood GX, d-Xyl, and most other reagents were purchased from Sigma-Aldrich (Darmstadt, Germany). Low-viscosity wheat arabinoxylan (Ara:Xyl = 38:62; LVWAX), acid-debranched wheat arabinoxylan (Ara:Xyl = 22:78; ADWAX), enzyme-debranched wheat arabinoxylan (Ara:Xyl = 30:70; EDWAX), rye arabinoxylan (Ara:Xyl = 38:62; RAX), galactomannan (carob; low viscosity), xyloglucan (tamarind), arabinan (sugar beet), β-glucan (barley; medium viscosity), xylobiose (X₂), xylotriose (X₃), xylotetraose (X₄), xylopentaose (X₅), xylohexaose (X₆), p-nitrophenyl-β-d-xylopyranoside (pNPX), p-nitrophenyl-β-d-xylobiose (pNPX₂), p-nitrophenyl-β-d-xylotriose (pNPX₃), and p-nitrophenyl-β-d-xylotetraose (pNPX₄) were all purchased from Megazyme (Bray, Ireland). Cellulose nanocrystals (nanocellulose) from cotton linters were prepared as previously described (56 (link)). Oligonucleotide primers were purchased from Eurogentec (Liège, Belgium) (Table 3).
The GenBank accession number for the clone containing Pm25 is HF548280.1, and the protein ID for Pm25 is CCO21036.1.