Cobas amplicor hbv monitor test kit

Manufactured by Roche

The Cobas Amplicor HBV monitor test kit is a laboratory equipment product used for the quantitative detection of hepatitis B virus (HBV) DNA in human plasma or serum samples. It provides a reliable and standardized method for monitoring HBV viral load in patients.

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Lab products found in correlation

Model 736, by Hitachi (2 mentions) Radioimmunoassay, by Abbott (1 mentions) Elecsys hbsag 2 quant assay, by Roche (1 mentions) Cobas taqman hcv test v2, by Roche (1 mentions)

3 protocols using cobas amplicor hbv monitor test kit

Genetic Factors Influencing HBsAg Seroclearance

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All patients were tested for hepatitis B or virological markers in the liver, including HBsAg was measured using radioimmunoassay with commercial kits (Abbott Laboratories, North Chicago), HBV DNA were measured by polymerase chain reaction (PCR) using the Cobas Amplicor HBV monitor test kit (Roche Diagnostics, Indianapolis, IN) and alanine transaminase (ALT) ALT using chemistry autoanalyzer (Model 736; Hitachi, Tokyo, Japan) using commercial reagents (Biomérieux, Marcy L’Etoile, France). HBsAg seroclearance were defined as loss of HBsAg in serum at least 6 months apart on 2 occasions and continued to absent up to the last visit. While without HBsAg seroclearance were defined as positive of HBsAg in serum for more than 6 months apart and continuously detected up to the last visit.
The SNPs we investigated in this present study were the replicated study from a GWAS applied in a Korean CHB population reported by Kim et al. Kim et al reported 3 SNPs (rs7944135, rs171941, rs6462008) associated with seroclearance of the HBsAg in Korean CHB patients. These 3 SNPs were confirmed to exist in a Taiwanese CHB population.

Irham L.M., Wong H.S., Perwitasari D.A., Chou W.H., Yang H.I, & Chang W.C. (2019). Single-nucleotide polymorphism of rs7944135 (macrophage-expressed gene 1) is associated with hepatitis B surface antigen seroclearance in chronic hepatitis B infection: A cohort study. Medicine, 98(51), e17936.

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Hepatitis Viral Serology and Quantification

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HBeAg and HBsAg serostatus were detected by radioimmunoassay (Abbott Laboratories). Anti-HCV was detected by enzyme immunoassay using a second-generation test kit (Abbott Laboratories). Serum ALT levels were measured by the serum chemistry autoanalyzer (Model 736, Hitachi Co.) using commercial reagents (Biomerieux). Serum HBV DNA levels were assayed by the COBAS Amplicor HBV monitor test kit (Roche Diagnostics). Serum HBsAg levels were quantified by the Elecsys HBsAg II Quant assay (Roche Diagnostics). HBV genotype was determined by melting curve analysis in participants with detectable serum HBV DNA levels.

Chu Y.J., Yang H.I., Hu H.H., Liu J., Lin Y.L., Chang C.L., Luo W.S., Jen C.L, & Chen C.J. (2023). HBV genotype-dependent association of HLA variants with the serodecline of HBsAg in chronic hepatitis B patients. Scientific Reports, 13, 359.

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Arsenic Exposure and Viral Hepatitis

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In an arseniasis area in southwestern Taiwan, most residents had used artesian deep-well water for >50 years from 1910-1970s. Arsenic content in these deep wells ranged from 350 to 1140 mg/L (median, 780 mg/L). The individual's cumulative arsenic exposure from the artesian well water was defined as S(CiÂDi), where Ci was the median well arsenic level of the village and Di was the duration of residency in the village. A detailed description of this cohort has been presented previously. 19 Serologic markers, including HBsAg and anti-HCV, were tested for 724 samples. Forty age-and gender-matched HBsAg-seropositive pairs with either cumulative arsenic exposure !14,000 mg/L Â years (median) or <14,000 were chosen for hepatitis B virus (HBV) DNA testing. The serum HBV DNA was quantified using polymerase chain reaction-based nucleic acid amplification (CobasAmplicorHBVmonitor test kit, Roche Diagnostics, Indianapolis, IN). Samples seropositive for anti-HCV were further examined for HCV RNA by polymerase chain reaction (Cobas TaqMan HCV test, v2.0, Roche Diagnostics, Indianapolis, IN). The detection limit for Cobas TaqMan HCV test was 25 IU/mL.

Hsu L.I., Wang Y.H., Hsieh F.I., Yang T.Y., Wen-Juei Jeng R., Liu C.T., Chen C.L., Hsu K.H., Chiou H.Y., Wu M.M, & Chen C.J. (2016). Effects of Arsenic in Drinking Water on Risk of Hepatitis or Cirrhosis in Persons With and Without Chronic Viral Hepatitis. Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association, 14(9).

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