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Api3200md triple quadrupole mass spectrometer

Manufactured by AB Sciex
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Sourced in United States

The API3200MD is a triple quadrupole mass spectrometer from AB Sciex. It is designed to perform sensitive and accurate quantitative analysis of small molecules in complex samples.

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Lab products found in correlation

Series liquid chromatograph, by Shimadzu (2 mentions)

2 protocols using api3200md triple quadrupole mass spectrometer

1

Quantitative bile acid profiling

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The bile acid profile was detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with the API3200MD triple quadrupole mass spectrometer (ABSciex, USA) and Shimadzu series liquid chromatograph (Shimadzu, Japan). The reagent kits were purchased from Shanghai ClinMeta Co., Ltd. The assay comprised 15 components: CA, GCA, TCA, CDCA, GCDCA, TCDCA, DCA, GDCA, TDCA, LCA, GLCA, TLCA, UDCA, GUDCA, TUDCA. The reagent kit was equipped with 12 isotope internal standards. Among them, due to isomer variant, DCA and CDCA share the same internal standard DCA-d4, GDCA and GCDCA share the same internal standard GDCA-d4, and TDCA and TCDCA share the same internal standard TDCA-d4. The remaining analytes are each equipped with a separate internal standard.
Xu R.H., Shen J.N., Lu J.B., Liu Y.J., Song Y., Cao Y., Wang Z.H, & Zhang J. (2024). Bile acid profiles and classification model accuracy for inflammatory bowel disease diagnosis. Medicine, 103(23).
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2

Bile Acid Profiling by LC-MS/MS

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The bile acid profile was detected using liquid chromatography-tandem mass spectrometry (LC–MS/MS) with the API3200MD triple quadrupole mass spectrometer (ABSciex, USA) and Shimadzu series liquid chromatograph (Shimadzu, Japan). The reagent kits were purchased from Shanghai ClinMeta Co., Ltd. The assay comprised 15 components: cholic acid (CA), glycocholic acid (GCA), taurocholic acid (TCA), chenodeoxycholic acid (CDCA), glycochenodeoxycholic acid (GCDCA), taurochenodeoxycholic acid (TCDCA), deoxycholic acid (DCA), glycodeoxycholic acid (GDCA), taurodeoxycholic acid (TDCA), lithocholic acid (LCA), glycolithocholic acid (GLCA), taurolithocholic acid (TLCA), ursodeoxycholic acid (UDCA), glycoursodeoxycholic acid (GUDCA), and tauroursodeoxycholic acid (TUDCA).
Xu R., Shen J., Song Y., Lu J., Liu Y., Cao Y., Wang Z, & Zhang J. (2024). Exploration of the application potential of serum multi-biomarker model in colorectal cancer screening. Scientific Reports, 14, 10127.
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