Sandwich ELISA assays were performed to quantify the albumin in cultures. The antibodies used were an anti-human Albumin IgG (Bethyl, Japan, capture antibody) and an anti-human Albumin IgG coupled with peroxidase (Bethyl, Japan, detection antibody). The plate was read at 490 nm using the iMark Microplate reader (Bio-Rad) after peroxidase revelation by H2O2/OPD mixture.
Anti human albumin igg
Manufactured by Fortis Life Sciences
Sourced in Japan
The Anti-Human Albumin IgG is a laboratory reagent used to detect and quantify human albumin in various samples. It is a purified immunoglobulin G (IgG) fraction that specifically binds to human albumin. This reagent can be used in analytical techniques such as immunoassays to measure albumin levels in biological samples.
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Lab products found in correlation
3 protocols using anti human albumin igg
1
Quantification of Albumin in Cell Cultures
2
ELISA Assay for Albumin Quantification
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To measure the production of albumin, we performed ELISA sandwich assays in a 96-well plate. The surface activation step was performed using anti-Human Albumin IgG (Bethyl, Japan) diluted at 1/1000 in a Tris buffer (0.1 M, pH = 8.0) overnight at 4°C. The passivation step was realized with gelatin (2 %) diluted in PBS and Tween 20 (0.1 %) and incubated for 1 hour. The samples were incubated overnight at 4°C. After washing, the second anti-Human Albumin IgG coupled with peroxidase (Bethyl, Japan) was incubated overnight at 4°C. The peroxidase activity was revealed by a mixture of H 2 O 2 and OPD in citrate buffer (0.05 mM, pH = 7.4).
The reaction was stopped with a sulfuric acid solution (4N) and the plate was read at 490nm (iMark Microplate reader, Bio-Rad).
The reaction was stopped with a sulfuric acid solution (4N) and the plate was read at 490nm (iMark Microplate reader, Bio-Rad).
3
Albumin Quantification by ELISA Sandwich Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To measure the production of albumin, we performed ELISA sandwich assays in a 96-well plate. The surface activation step was performed using anti-Human Albumin IgG (Bethyl, Japan) diluted at 1/1000 in a Tris buffer (0.1 M, pH = 8.0) overnight at 4°C. The passivation step was realized with gelatin (2 %) diluted in PBS and Tween 20 (0.1 %) and incubated for 1 hour. The samples were incubated 3h at room temperature. After washing, the second anti-Human Albumin IgG coupled with peroxidase (Bethyl, Japan) was incubated at room temperature for 3h. The peroxidase activity was revealed by a mixture of H 2 O 2 and OPD in citrate buffer (0.05 mM, pH = 7.4). The reaction was stopped with a sulfuric acid solution (4N) and the plate was read at 490nm (iMark Microplate reader, Bio-Rad).
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