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2 protocols using 17 β estradiol e2

1

Estrogen receptor agonist and inhibitors

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17-β-estradiol (E2), a general oestrogen receptors agonist, was purchased from Abcam (ab120657); the selective GPR30 agonist G1, the selective GPR30 inhibitor G15 and the specific PI3K inhibitor wortmannin were purchased from Sigma-Aldrich (G6798; G6748; W1628;)
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2

Estrogen Receptor Signaling Pathways

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17β-estradiol (E2) was purchased from Abcam (US). The MEK1/2 inhibitor U0126, the Src inhibitor PP2, the EGFR inhibitor AG1478 were purchased from Sigma Chemical Co (US). pSrc-Y416, pSrc-Y527, βtubulin, p-ERK, ERK, p-P38 and P38 antibodies purchased from Cell Signaling Technology (US); Anti-ER-α and ER-β purchased from abcam; CyclinD1, CyclinE, CyclinB, GAPDH, CDK4, EGFR and Src antibodies purchased from Proteintech (Wuhan, China); KI67 antibody purchased from Zhongshan Goldenbridge Biotechnology (Beijing, China). anti-ER-α36 antibody, ER-α36 expression vector and ER-α36 shRNA were from Dr. Wang ZY (Creighton University Medical School).
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