RNA and DNA were extracted concomitantly using the quick-DNA/RNA Magbead extraction kit (Zymo). Briefly, samples were lysed in 800 μL of DNA/RNA shield (Zymo) using TissueLyser LT (Qiagen), and then, the lysates were incubated with Proteinase K for 30 minutes at room temperature and frozen at −80°C. Bead-based RNA and DNA extraction from 100 μL of lysates was performed automatically using the Pureprep 96 Nucleic Acid Purification System (Magtivio). RNA and DNA concentrations were measured using the Qubit 2.0 fluorometer and the Qubit RNA (or DNA) High Sensitivity Kit (ThermoFisher).
Quick dna rna magbead extraction kit
Manufactured by Zymo Research
Sourced in Switzerland
The Quick DNA/RNA MagBead Extraction Kit is a laboratory equipment product designed for the rapid and efficient extraction of DNA and RNA from a variety of sample types using magnetic bead technology. The kit provides a streamlined protocol and optimized reagents to facilitate the isolation of nucleic acids.
Automatically generated - may contain errors
3 protocols using quick dna rna magbead extraction kit
1
Automated Dual Nucleic Acid Extraction
2
DNA Extraction from Fresh Specimens
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total DNA was extracted from fresh specimens on the Freedom EVO 100 (Tecan, Mannedorf, Switzerland) using the Quick DNA/RNA MagBead Extraction Kit (Zymo Research, Irvine, California, USA), as per the manufacturer’s instructions. DNA extracts were stored at −20°C, and the original lesion and non-lesion study samples were stored at −70°C.
3
DNA Extraction from Fresh Specimens
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total DNA was extracted from fresh specimens on the Freedom EVO 100 (Tecan, Mannedorf, Switzerland) using the Quick DNA/RNA MagBead Extraction Kit (Zymo Research, Irvine, California, USA), as per the manufacturer’s instructions. DNA extracts were stored at −20°C, and the original lesion and non-lesion study samples were stored at −70°C.
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