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Fibrinogen

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Fibrinogen is a plasma glycoprotein that plays a crucial role in the blood clotting process. It is an essential component of the coagulation cascade and is involved in the formation of fibrin, the main structural protein in blood clots. Fibrinogen is measured and analyzed in various laboratory settings to assess an individual's blood clotting ability and to aid in the diagnosis and monitoring of certain medical conditions.

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Lab products found in correlation

Plasminogen, by Enzyme Research (6 mentions) Fibrinogen, by Thermo Fisher Scientific (2 mentions) Fibrinogen, by Merck Group (2 mentions) Hlmvec, by PromoCell (2 mentions) Ebm 2, by Lonza (2 mentions)

2 protocols using fibrinogen

1

Fibrin Formation and Lysis Kinetics

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Thrombin (5 nM final; Sekisui Diagnostics) was mixed with fibrinogen (2-9 g/L final; ThermoFisher Scientific, Waltham, MA), preincubated with either FXII or FXIIa (10-40 μg/mL final; both from Sekisui Diagnostics) in a total volume of 25 μL of 0.1 M imidazole buffer in a clear, flat-bottomed, 96-well plate. Fibrin formation was initiated by the addition of 20 μL of 20 mM CaCl2. To measure fibrinolysis, tPA (0.1 µg/mL final) and plasminogen (20 µg/mL final; Enzyme Research Laboratories) were added to the clotting solution. Turbidity was monitored as described earlier. In some experiments, FXIIa (40 μg/mL final) was preincubated with corn trypsin inhibitor (CTI; 0.01 U/mL final; Sekisui Diagnostics) before mixing with fibrinogen. FXII and FXIII contaminations were not detected in fibrinogen preparation by means of western blot analysis and enzyme-linked immunosorbent assay.
Wygrecka M., Birnhuber A., Seeliger B., Michalick L., Pak O., Schultz A.S., Schramm F., Zacharias M., Gorkiewicz G., David S., Welte T., Schmidt J.J., Weissmann N., Schermuly R.T., Barreto G., Schaefer L., Markart P., Brack M.C., Hippenstiel S., Kurth F., Sander L.E., Witzenrath M., Kuebler W.M., Kwapiszewska G, & Preissner K.T. (2022). Altered fibrin clot structure and dysregulated fibrinolysis contribute to thrombosis risk in severe COVID-19. Blood Advances, 6(3), 1074-1087.
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2

Endothelial Cell Response to Plasma Treatments

Cited 2 times
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Human lung microvascular endothelial cells (HLMVEC; PromoCell) were grown to confluence in endothelial basic medium-2 (EBM-2; Lonza) supplemented with 10% fetal bovine serum (FBS), human recombinant epidermal growth factor, human recombinant insulin-like growth factor-1, human basic fibroblast growth factor, vascular endothelial growth factor, hydrocortisone, ascorbic acid, heparin, gentamicin, and amphotericin B. Endothelial cells (passages 6–8) were incubated with EBM-2 containing 2% FBS prior to experiments. Lactated Ringer’s solution (LR), fresh frozen plasma (FFP), and fibrinogen depleted plasma (FDP; Sekisui Diagnostics, Germany), were diluted in media to 10% as we have previously demonstrated this to be optimal for endothelial cell function (4 (link), 14 (link)). fibrinogen-deficient plasma was purified using human thrombin but still contains a small amount of fibrinogen (0.08 mg/ml). fibrinogen (F3879, Sigma-Aldrich) contains 50-70% protein (≥80% of protein is clottable) was added to medium at 2.5, 5.0 and 10.0 mg/ml.
Wu F, & Kozar R.A. (2019). Fibrinogen protects against barrier dysfunction through maintaining cell surface syndecan-1 in-vitro. Shock (Augusta, Ga.), 51(6), 740-744.
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