Lysogeny broth lb

Bacterial strains and plasmids used in this study are listed in Table 1. Acidovorax citrulli strains were routinely grown at 28 °C in nutrient broth (NB; Difco Laboratories, Detroit, MI, USA), NA (NB containing 15 g/L agar) or Lysogeny Broth (LB; Difco Laboratories). XVM2 minimal medium, which resembles to some extent the plant apoplast environment [26 (link)], was used for growth curve experiments and for biofilm formation and lipolytic assays (see below). Escherichia coli strains were cultured in LB at 37 °C. Antibiotics were added at the following concentrations: ampicillin (Ap), 100 μg/mL; kanamycin (Km), 50 μg/mL; and gentamicin (Gm), 10 or 30 μg/mL (for E. coli and A. citrulli, respectively).

The plasmids and bacterial strains used in this study are listed in Supplementary Table S1, and the primers used for plasmid constructions in Supplementary Table S2. B. subtilis 168 and Escherichia coli TG1 were grown with vigorous agitation in Lysogeny broth (LB; Difco laboratories) at 37°C. Where appropriate, the growth medium was supplemented with antibiotics: ampicillin 100 μg/ml, spectinomycin 100 μg/ml, chloramphenicol 5 μg/ml (B. subtilis) or 10 μg/ml (E. coli).

The bacterial strains, plasmids, and oligonucleotides used in the study are listed in Tables S1 and S2 in the supplemental material. For plasmid construction, E. coli DH5α served as a host. E. coli strains were grown in Lysogeny broth (LB) (Difco Laboratories) at 37°C with shaking. E. coli strains were grown with the following antibiotics as needed: chloramphenicol (20 μg ml⁻¹), erythromycin (100 μg ml⁻¹), and spectinomycin (50 μg ml⁻¹). E. faecalis strains were grown in brain heart infusion broth (BHI; Difco Laboratories) or M9 minimal medium (26 (link)) at 37°C without shaking. E. faecalis strains were grown with the following antibiotics as needed: erythromycin (100 μg ml⁻¹ final concentration for plasmid markers, 10 μg ml⁻¹ for chromosomal markers), fusidic acid (25 μg ml⁻¹), rifampin (200 μg ml⁻¹), spectinomycin (1,000 μg ml⁻¹ for plasmid markers, 250 μg ml⁻¹ for chromosomal markers), streptomycin (1,000 μg ml⁻¹), tetracycline (10 μg ml⁻¹), and chloramphenicol (10 μg ml⁻¹). Antibiotics were obtained from Sigma-Aldrich.