Pd184352

Manufactured by LC Laboratories

Sourced in United States

PD184352 is an organic compound that functions as a MEK (Mitogen-Activated Protein Kinase Kinase) inhibitor. It is commonly used in biological research applications.

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Lab products found in correlation

Sb203580, by LC Laboratories (2 mentions) Williams e medium, by Merck Group (2 mentions) Sp600125, by LC Laboratories (2 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (2 mentions) Cisplatin, by Apexbio (1 mentions) Sp600125, by Apexbio (1 mentions) Noxa 114c307.1, by Thermo Fisher Scientific (1 mentions) Mcl 1, by Enzo Life Sciences (1 mentions) P erk, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Rapamycin, by Merck Group (1 mentions) Sb216763, by Merck Group (1 mentions) P akt ser473, by Cell Signaling Technology (1 mentions) Usp9x, by Santa Cruz Biotechnology (1 mentions) Ly290024, by Merck Group (1 mentions) P mtor, by Cell Signaling Technology (1 mentions) P70s6k, by Cell Signaling Technology (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Sorafenib, by LC Laboratories (1 mentions) U0126, by LC Laboratories (1 mentions)

4 protocols using pd184352

Investigating Cisplatin-Induced Cell Stress

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Cisplatin and SP600125 were purchased from ApexBio (Houston, TX, USA). SB203580 and PD184352 were purchased from LC Laboratories (Woburn, MA, USA). Cisplatin was dissolved in PBS and other reagents were dissolved in dimethyl sulfoxide (Hall et al., 2014). The following antibodies were used: cleaved‐PARP (D64E10), ATF4, CHOP and GAPDH from Cell Signaling Technology (Danvers, MA, USA); Noxa (114C307.1) from Thermo Fisher Scientific (Waltham, MA, USA); MCL‐1 from Enzo Life Sciences (Farmingdale, NY, USA); ATF3, ATF4, pERK, ERK1, and ERK2 from Santa Cruz Biotechnology (Santa Cruz, CA, USA); ATF4 from GeneTex (Irvine, CA, USA).

Sharma K., Vu T., Cook W., Naseri M., Zhan K., Nakajima W, & Harada H. (2018). p53‐independent Noxa induction by cisplatin is regulated by ATF3/ATF4 in head and neck squamous cell carcinoma cells. Molecular Oncology, 12(6), 788-798.

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Signaling Pathway Regulation Assay

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ATRA, rapamycin, LY290024, SB216763 and cycloheximide (CHX) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). U0126, PD184352 and Sorafenib were obtained from LC Laboratories (Woburn, MA, USA). Antibody to Mcl-1, ERK1, Bcl-2, β-actin, and USP9X were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA); to PARP was from BD Biosciences (San Diego, CA, USA ); to DR5 was from ProSci, Inc. (Poway, CA, USA); to p-MEK (Ser^217/221), p-ERK (Thr²⁰²/Tyr²⁰⁴), Akt, p-Akt (Ser⁴⁷³), p-mTOR, p70S6K, p-p70S6K (Thr³⁸⁹), p-p70S6K (Thr⁴²¹/Ser⁴²⁴), p-p90RSK (Ser³⁸⁰), p90RSK, p-rpS6 (Ser^235/236), rpS6, GSK-3β, p-GSK-3β (Ser⁹), caspase-8, -9, -3 and Trail were from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Wang R., Xia L., Gabrilove J., Waxman S, & Jing Y. (2015). Sorafenib inhibition of Mcl-1 accelerates ATRA induced apoptosis in differentiation responsive AML cells. Clinical cancer research : an official journal of the American Association for Cancer Research, 22(5), 1211-1221.

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Cellular Signaling Pathway Modulation

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Williams’ Medium E, 4-phenylbutyrate acid (4-PBA), and dimethy-sulfoxide (DMSO) were obtained from Sigma-Aldrich (St. Louis, MO). Fetal bovine serum (FBS) was purchased from Atlanta Biologicals (Lawrenceville, GA). Antibiotic-antimycotic was obtained from Life Technologies (Grand Island, NY). PureCol Bovine Collagen Solution was from Advanced BioMatrix (San Diego, CA). Leflunomide was purchased from Enzo Life Sciences (Farmingdale, NY). SP600125 (JNK inhibitor) and PD184352 (ERK1/2 inhibitor) were from LC laboratories (Woburn, MA).

Ren Z., Chen S., Qing T., Xuan J., Couch L., Yu D., Ning B., Shi L, & Guo L. (2017). Endoplasmic reticulum stress and MAPK signaling pathway activation underlie leflunomide-induced toxicity in HepG2 Cells. Toxicology, 392, 11-21.

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Cell Signaling Pathway Modulation

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Williams’ Medium E, nefazodone, trazodone, buspirone, penicillin, streptomycin, salubrinal, and dimethysulfoxide (DMSO) were from Sigma-Aldrich (St. Louis, Missouri). Fetal bovine serum (FBS) was obtained from Atlanta Biologicals (Lawrenceville, Georgia). 4-Phenylbutyrate (4-PBA) was from BioVision (Milpitas, California). Blasticidin hydrochloride was purchased from Life Technologies (Grand Island, New York). SB203580 (p38 inhibitor), SP600125 (JNK inhibitor), and PD184352 (ERK1/2 inhibitor) were from LC Laboratories (Woburn, Massachusetts).

Ren Z., Chen S., Zhang J., Doshi U., Li A.P, & Guo L. (2016). Endoplasmic Reticulum Stress Induction and ERK1/2 Activation Contribute to Nefazodone-Induced Toxicity in Hepatic Cells. Toxicological sciences : an official journal of the Society of Toxicology, 154(2), 368-380.

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