Each mouse was left to explore freely for 5 min in a maze consisting of the following four perpendicular arms (40 × 7 cm): two open arms (1 cm high) and two closed arms (16 cm high), in a well lit room. Behavior was recorded using a computer-linked video camera (The Imaging Source) located above the maze. The total time spent in the open arms was measured using EthoVision XT software (Noldus).
Computer linked video camera
Manufactured by Imaging Source
The Computer-linked video camera is a device that captures and transmits visual information to a connected computer. It functions as an input device, allowing the computer to receive and process real-time video data.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using computer linked video camera
1
Open Field Exploration Behavior
2
Social Interaction in Mice Across Development
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At P21, test pups were habituated to the arena for 3 min. Subsequently, WT females in estrus, unfamiliar to the pups, were added to the cage, and both mice were allowed to interact with each other for another 3 min in a well lit room. The interaction between the pups and the females was recorded using a computer-linked video camera (The Imaging Source) located above the arena. Videos were manually scored, and interaction was recorded when both mice were within 2 cm of each other, not including tail–tail interactions.
At P60, only female mice were tested for social interaction. In this case the unknown WT females were not required to be in estrus.
To determine which females were in estrus, vaginal smears were stained with Giemsa solution (Polysciences; Caligioni, 2009 ) before the experiment.
At P60, only female mice were tested for social interaction. In this case the unknown WT females were not required to be in estrus.
To determine which females were in estrus, vaginal smears were stained with Giemsa solution (Polysciences; Caligioni, 2009 ) before the experiment.
3
Open Field Locomotion Analysis
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Open field behavioral analysis was performed on two consecutive days, using the first day to habituate the mice to the new environment. Mice were allowed to explore freely, in the dark, for 20 min, in an open field arena (40 cm x 40 cm). Spontaneous locomotion was recorded using a computer-linked video camera (The Imaging Source) located above the arena and an infrared illumination box (Tracksys) located underneath the arena. The EthoVision XT software (Noldus) was used to analyze total distance travelled, distance travelled in intervals of 5 min and time spent in the center of the arena. The center of the arena was defined as the area separated from the wall by 5 cm or more.
4
Social Interaction in Mice: Developmental Dynamics
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At P21, test pups were habituated to the arena for 3 min. Subsequently, WT females in estrous, unfamiliar to the pups, were added to the cage and both mice were allowed to interact with each other for another 3 min in a well-lit room. The interaction between the pups and the females was recorded using a computer-linked video camera (The Imaging Source) located above the arena. Videos were manually scored, and interaction recorded when both mice were within 2 cm of each other, not including tail-tail interactions.
At P60, only female mice were tested for social interaction. In this case the unknown WT females were not required to be in oestrus.
To determine which females were in oestrus, vaginal smears were stained with Giemsa solution (Polysciences inc.) (Caligioni 2009) prior to the experiment.
At P60, only female mice were tested for social interaction. In this case the unknown WT females were not required to be in oestrus.
To determine which females were in oestrus, vaginal smears were stained with Giemsa solution (Polysciences inc.) (Caligioni 2009) prior to the experiment.
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