Bovine heart extract

Manufactured by Avanti Polar Lipids

Bovine heart extract is a laboratory reagent derived from the heart tissue of cattle. It contains a complex mixture of lipids, proteins, and other biological molecules found naturally in the bovine heart. The extract is commonly used in various biochemical and cell culture applications as a source of essential nutrients and signaling molecules.

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Lab products found in correlation

Hplc grade ammonium acetate, by Thermo Fisher Scientific (1 mentions) Equisplash, by Avanti Polar Lipids (1 mentions) Dichloromethane, by Merck Group (1 mentions) Internal standards, by Avanti Polar Lipids (1 mentions)

2 protocols using bovine heart extract

Comprehensive Lipidomic Sample Preparation

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LCMS Optima-grade methylene chloride (DCM), methanol (MeOH), acetonitrile (ACN), and water (H2O) were purchased from Fisher Scientific; Hampton, NH, USA. LCMS-grade isopropanol (IPA) was purchased through HoneyWell (Charlotte, NC, USA). HPLC-grade ammonium acetate was acquired from Fisher Scientific. EquiSplash (Avanti Polar Lipids, Inc.; Birmingham, AL, USA), used as an internal standard, was a mixture of phosphatidylcholine-d7, PC(15:0/18:1-d7); lysophosphatidylcholine-d7, LPC(15:0/18:1-d7); phosphatidylethanolamine-d7, PE(15:0/18:1-d7); lysophosphatidylethanolamine-d7, LPE(15:0/18:1-d7); phosphatidylglycerol-d7, PG(15:0/18:1-d7); phosphatidylinositol-d7, PI(15:0/18:1-d7); phosphatidylserine-d7, PS(15:0/18:1-d7); triacylglyceride-d7, TAG(15:0/18:1-d7/15:0); diacylglyceride-d7, DAG(15:0/18:1-d7); monoacylglyceride-d7, MAG(18:1-d7); cholesterol ester-d7, CE(18:1-d7); sphingomyelin-d9, SM(d18:1/18:1-d9); and ceramide-d7 (d18:1-d7/15:0). The internal standard mix was diluted 100× with 1:1 DCM:MeOH. Bovine heart extract was purchased from Avanti Polar Lipids, Inc.

Schramm W.C., Bala N., Arekar T., Malik Z., Chacko K.M., Lewis R.L., Denslow N.D., Scindia Y, & Alli A.A. (2024). Enrichment of Bioactive Lipids in Urinary Extracellular Vesicles and Evidence of Apoptosis in Kidneys of Hypertensive Diabetic Cathepsin B Knockout Mice after Streptozotocin Treatment. Biomedicines, 12(5), 1038.

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Lipid Standards for Mass Spectrometry

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Dichloromethane (Sigma-Aldrich), methanol and ammonium acetate (Fluka), and water (Burdick and Jackson) were LC-MS grade. Teflon coated caps and glass tubes were from VWR. 12 mm x 32 mm amber vials were from Thermo Scientific and 9 mm threaded caps were from Wheaton Inc. Internal standards (Avanti Polar Lipids, Inc.) were used for monitoring lipid class recoveries for: TAGs, D5-TAG (1,3 dipentadecanoyl-2-(9z-octadecanoyl)-sn-glycerol ²H₅, F_w 810.34), GPLs, PC 17:0/14:1 (1-heptadecanoyl-2-(9z-tetradecanoyl)-sn-glycerol-phospholcholine, F_w 718.00), sphingosines, SM 18:1;2/17:0 (n-heptadecanoyl-D-erythro-sphingosylphosphocholine, F_w 717.07). Bovine heart extract (Avanti Polar Lipids, Inc.) at 0.25-2.5 ng/ml in methanol/Dichloromethane/ammonium acetate infusion solvent was used for mass (MS and MS/MS) calibration.

Liaw L., Prudovsky I., Koza R.A., Anunciado-Koza R.V., Siviski M.E., Lindner V., Friesel R.E., Rosen C.J., Baker P.R., Simons B, & Vary C.P. (2016). Lipid profiling of in vitro cell models of adipogenic differentiation: relationships with mouse adipose tissues. Journal of cellular biochemistry, 117(9), 2182-2193.

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