All cell lines (A549, H1299, H1650, SPC‐A1, H1975, H358, PC9, and human bronchial epithelial cell [HBE]) were purchased from Shanghai Institutes for Biological Science (Shanghai, China). A549, H1650, SPC‐A1, H1975, H358, and HBE were cultured in DMEM medium (KeyGene, Nanjing, China); H1299 and PC9 were cultured in RPMI1640 medium (KeyGene), supplemented with 10% FBS with 100 μg/mL penicillin and 100 mg/mL streptomycin included. All cell lines were grown in humidified air at 37°C with 5% CO2. Cell cultures were occasionally tested for mycoplasma (last tested December 2018). Authentication of cells was verified by short tandem repeat DNA profiling within 6 months, and cells used in experiments were within 10 passages from thawing. Cell proliferation was examined using a CCK‐8 Kit (Roche Applied Science). Colony formation assays were performed to monitor LUAD cell cloning capability. Flow cytometer (FACScan, BD Biosciences) equipped with CellQuest software (BD Biosciences) was used to detect apoptosis level.
H1299
Manufactured by Keygene
Sourced in China
The H1299 is a cell line used in laboratory research. It is a human non-small cell lung cancer cell line derived from the lymph node metastasis of a lung cancer patient.
Automatically generated - may contain errors
Lab products found in correlation
H1975, by Keygene (2 mentions)
Facscan, by BD (1 mentions)
Cellquest software, by BD (1 mentions)
Spc a1, by Keygene (1 mentions)
Dmem medium, by Keygene (1 mentions)
Rpmi 1640 medium, by Keygene (1 mentions)
Cck 8 kit, by Roche (1 mentions)
Rpmi 1640, by Keygene (1 mentions)
Dulbecco modified eagle medium (dmem), by Keygene (1 mentions)
2 protocols using h1299
1
Lung Cancer Cell Line Characterization
2
Glucose Starvation in Human Lung Cells
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Human bronchial epithelial cell (HBE) and lung adenocarcinoma cell lines (A549, H1299, PC9 and H1975) were purchased from Shanghai Institutes for Biological Science (Shanghai, China). HBE and H1975 were cultivated in DMEM (KeyGene, Nanjing, China), while A549, H1299 and PC9 cells were cultivated in RPMI-1640(KeyGene, Nanjing, China), all supplemented with 10% FBS (KeyGene, Nanjing, China) and cultured in a standard 37℃ incubator. For glucose starvation experiments, cells were cultured in medium with different concentrations of glucose.
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