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Labmaster system

Manufactured by TSE Systems
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Sourced in Germany

The LabMaster System is a versatile lab equipment designed for precise measurements and data collection. It features advanced sensors and a user-friendly interface, allowing for efficient data processing and analysis. The core function of the LabMaster System is to provide accurate and reliable data to support various laboratory experiments and research applications.

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Lab products found in correlation

Echomri, by Echo Medical Systems (9 mentions) Phenomaster, by TSE Systems (4 mentions) Metabolic chambers, by TSE Systems (4 mentions) Phenomaster software, by TSE Systems (4 mentions) Minispec mq7.5 live mice analyzer, by Bruker (3 mentions) Accu chek compact plus, by Roche Diabetes Care (2 mentions) Bio plex pro mouse diabetes immunoassay, by Bio-Rad (2 mentions) Metabolic cages, by TSE Systems (2 mentions) Phenomaster system, by TSE Systems (1 mentions) C57bl 6 mice, by Orient Bio (1 mentions) Paraoxon, by Merck Group (1 mentions) Ab83390, by Abcam (1 mentions) Videomot2, by TSE Systems (1 mentions) Bp 98a, by Softron (1 mentions) Videomot 2 tracking software, by TSE Systems (1 mentions) Opto m3, by Columbus Instruments (1 mentions) Opto m3 animal activity meter, by Columbus Instruments (1 mentions)

64 protocols using labmaster system

1

Indirect Calorimetry for Energy Expenditure

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Indirect calorimetry was performed in fully automatic metabolic cages (LabMaster System, TSE Systems, Bad Homburg, Germany) during the ninth week of treatment. After 1 day of acclimatization, O2 consumption, CO2 production and caloric intake were measured for 3 consecutive days42 (link). Total EE was estimated from the VO2 and resting energy requirement. Carbohydrate oxidation was calculated using the formula ((4.585*VCO2)−(3.226*VO2))*4, in which the 4 represents the conversion from mass per time unit to kcal per time unit43 (link). Similarly, fat oxidation was calculated using the formula ((1.695*VO2)−(1.701*VCO))*9. Values were normalized to lean mass. Physical activity was monitored using infrared sensor frames. The first 12 h directly after injection with CL316243 or vehicle (‘Day of treatment’) were analysed and compared with the same 12-h period 24 h later (‘Day after treatment’).
Berbée J.F., Boon M.R., Khedoe P.P., Bartelt A., Schlein C., Worthmann A., Kooijman S., Hoeke G., Mol I.M., John C., Jung C., Vazirpanah N., Brouwers L.P., Gordts P.L., Esko J.D., Hiemstra P.S., Havekes L.M., Scheja L., Heeren J, & Rensen P.C. (2015). Brown fat activation reduces hypercholesterolaemia and protects from atherosclerosis development. Nature Communications, 6, 6356.
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2

Metabolic Flexibility Measurement Protocol

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After 12 weeks on the experimental diets, indirect calorimetric measurements were performed using the LabMaster System (TSE Systems) as previously described (21 (link)). The respiratory exchange ratio (RER) was measured as the ratio of O2 inhalation to CO2 production, averaged over a 12-h period. Metabolic flexibility was defined as the absolute change in RER between successive light and dark cycles. Energy expenditure, activity and food consumption were also quantified.
Regnier S.M., Kirkley A.G., Ruiz D., Kamau W., Wu Q., Kannan K, & Sargis R.M. (2017). Diet-dependence of metabolic perturbations mediated by the endocrine disruptor tolylfluanid. Endocrine Connections, 7(1), 159-168.
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3

Comprehensive Metabolic Assessment in Mice

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Locomotor activity, oxygen consumption and carbon dioxide production were simultaneously measured in individually housed mice with a LabMaster system (TSE Systems). Mice were acclimatised for 2 days, and data were collected for 2 days and analysed. The light and feeding conditions were consistent with those in the home cages.
Chen J., Xu X., Li Y., Li F., Zhang J., Xu Q., Chen W., Wei Y, & Wang X. (2020). Kdm6a suppresses the alternative activation of macrophages and impairs energy expenditure in obesity. Cell Death and Differentiation, 28(5), 1688-1704.
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4

Gas Exchange Measurement in Mice

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After a 2 day acclimatization period, O2 and CO2 gas exchange measurements were obtained for a 4 day period from each mouse using the open circuit chambers Labmaster system (TSE Systems, Germany).
Banhos Danneskiold-Samsøe N., Sonne S.B., Larsen J.M., Hansen A.N., Fjære E., Isidor M.S., Petersen S., Henningsen J., Severi I., Sartini L., Schober Y., Wolf J., Nockher W.A., Wolfrum C., Cinti S., Sina C., Hansen J.B., Madsen L., Brix S, & Kristiansen K. (2019). Overexpression of cyclooxygenase-2 in adipocytes reduces fat accumulation in inguinal white adipose tissue and hepatic steatosis in high-fat fed mice. Scientific Reports, 9, 8979.
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5

Circadian Behavior and Metabolism Monitoring

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The LabMaster system (TSE Systems, Chesterfield, MO) was used to measure the circadian pattern of locomotor activity and ingestion behavior as well as indirect calorimetry. Mice were placed in mock (non-recording) LabMaster cages for 1 week prior to testing to acclimate to the novel environment. After the acclimation period, the mice were transferred to recording cages at 8am. Data are recorded starting at 6am the following day, for 3 consecutive days. Animals received water and food ad libitum and remained undisturbed by the investigator during observation. The LabMaster system consists of a combination of feeding and drinking sensors, a photobeam-based activity monitoring system, and a calorimetry system to determine O2 consumption, CO2 production, and respiratory exchange ratio (volume CO2/ volume O2).
Bachstetter A.D., Webster S.J., Tu T., Goulding D.S., Haiech J., Watterson D.M, & Van Eldik L.J. (2014). Generation and Behavior Characterization of CaMKIIβ Knockout Mice. PLoS ONE, 9(8), e105191.
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6

Indirect Calorimetry in Offspring Mice

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Food consumption was registered daily, and body weight of each male offspring mouse was registered weekly for the 24-week period, always at the same time of the day during the middle of the light period. Indirect calorimetric measurements were carried out with the LabMaster system (TSE Systems, Midland, MI) in a separate subset of SF offspring and SC offspring at the Mouse Metabolic Core Facility to allow monitoring of VO2, VCO2, and respiratory exchange ratio. VO2, VCO2, and respiratory exchange ratio were recorded for 7 days during the 7th week of life, and gain was recorded during the 23rd to 24th weeks of life.
Khalyfa A., Mutskov V., Carreras A., Khalyfa A.A., Hakim F, & Gozal D. (2014). Sleep Fragmentation During Late Gestation Induces Metabolic Perturbations and Epigenetic Changes in Adiponectin Gene Expression in Male Adult Offspring Mice. Diabetes, 63(10), 3230-3241.
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7

Calorimetry of SOD1 ALS Mice

Cited 2 times
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Total energy expenditure of treated and untreated 120 day‐old SOD1G93A and WT littermate mice (n ≥ 6 for each experimental group) was measured through an indirect calorimetry system (TSE PhenoMaster/LabMaster System, Germany) at constant air flow of 0.35 L min−1. Measures were carried out at intervals of 20 min for each mouse, starting at 7:00 PM and ending automatically after 48 h or 72 h (12 h dark–light phase comparison). Mice were assessed under standard nutritional conditions (i.e. standard diet‐fed) and food/water intake and locomotor activity were continuously monitored (Giacovazzo et al., 2018 (link); Scaricamazza et al., 2020 (link); Steyn et al., 2020 (link)).
Scaricamazza S., Salvatori I., Amadio S., Nesci V., Torcinaro A., Giacovazzo G., Primiano A., Gloriani M., Candelise N., Pieroni L., Loeffler J., Renè F., Quessada C., Tefera T.W., Wang H., Steyn F.J., Ngo S.T., Dobrowolny G., Lepore E., Urbani A., Musarò A., Volonté C., Ferraro E., Coccurello R., Valle C, & Ferri A. (2022). Repurposing of Trimetazidine for amyotrophic lateral sclerosis: A study in SOD1G93A mice. British Journal of Pharmacology, 179(8), 1732-1752.
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8

Comprehensive Metabolic Assessment in Mice

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Three-month-old male mice were acclimated in metabolic chambers (TSE Systems) for 4 d before the start of the recordings. Mice were continuously recorded for 3 d with the following measurements being taken every 30 min: water intake, food intake, ambulatory activity (in X and Z axes), and gas exchange (O2 and CO2) (using the TSE LabMaster system). VO2, VCO2, and energy expenditure were calculated according to the manufacturer's guidelines (PhenoMaster Software, TSE Systems). Body composition was measured utilizing MRI (EchoMRI).
Kim1 J.G., Suyama S., Koch M., Jin S., Argente-Arizon P., Argente J., Liu Z.W., Zimmer M.R., Jeong J.K., Szigeti-Buck K., Gao Y., Garcia-Caceres C., Yi C.X., Salmaso N., Vaccarino F.M., Chowen J., Diano S., Dietrich M.O., Tschöp M.H, & Horvath T.L. (2014). Leptin signaling in GFAP-expressing adult glia cells regulates hypothalamic neuronal circuits and feeding. Nature neuroscience, 17(7), 908-910.
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9

Metabolic Cage Analysis of Mice

Cited 1 time
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During the third week of treatment, mice (n = 8 per group) were placed in fully automatic metabolic cages (LabMaster System, TSE Systems, Bad Homburg, Germany) to assess energy expenditure and physical activity levels. After 20 h of acclimatization, oxygen uptake (V˙ O2) and carbon dioxide production (V˙ CO2) were measured for five consecutive days. Total energy expenditure (EE) was calculated from V˙ O2 and V˙ CO2 using the Weir equation [35 (link)]. Fat and glucose oxidation rates were calculated from V˙ O2 and V˙ CO2, as described previously [36 (link)]. Physical activity was measured using infrared sensor frames.
Kuipers E.N., van Dam A.D., Held N.M., Mol I.M., Houtkooper R.H., Rensen P.C, & Boon M.R. (2018). Quercetin Lowers Plasma Triglycerides Accompanied by White Adipose Tissue Browning in Diet-Induced Obese Mice. International Journal of Molecular Sciences, 19(6), 1786.
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10

Metabolic Profiling of Mice Using LabMaster

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Metabolic studies were performed using the LabMaster system (TSE Systems, Bad Homburg, Germany) [15 (link)]. All mice used were acclimatized in metabolic cages for 48 h before measurements of each parameters with ad libitum access to food and drink. VCO2 and VO2 were determined using O2 and CO2 sensors calibrated against a standard gas mix containing defined quantities of O2 and CO2. The ratio between VCO2 and VO2 indicates the respiratory exchange ratio (RER) [15 (link)]. Animal activity was expressed as all of the movement recorded across the X and Y axes [16 (link)]. Daily food and drink intake were monitored by weighing food hoppers and drink bottles. All the measurements were taken every 15 min for a total of 24 h.
Arriga R., Pacifici F., Capuani B., Coppola A., Orlandi A., Scioli M.G., Pastore D., Andreadi A., Sbraccia P., Tesauro M., Daniele N.D., Sconocchia G., Donadel G., Bellia A., Della-Morte D, & Lauro D. (2019). Peroxiredoxin 6 Is a Key Antioxidant Enzyme in Modulating the Link between Glycemic and Lipogenic Metabolism. Oxidative Medicine and Cellular Longevity, 2019, 9685607.
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