Bacteria and diluted blood samples (~15% hematocrit) filled in a 5 mL syringe were pumped through the spiral device using a syringe pump (PHD 2000, Harvard Apparatus, USA) while sheath fluid (1× PBS supplemented with 0.1% BSA) was filled in a 60 mL syringe and pumped into the device with a separate syringe pump (PHD 2000, Harvard Apparatus, USA). The flow rate ratio between the sample and sheath flow was fixed at ~1:10 to form a tight sample stream at the outer wall. The microchannels were mounted on an inverted phase contrast microscope (Olympus IX71) equipped with a Hamamatsu Model C4742-80-12AG CCD camera (Hamamatsu Photonics, Japan) for imaging of fluorescent bacteria. A high speed CCD camera (Phantom v9, Vision Research Inc., USA) was also used to capture videos of inertially-focused blood cells at the channel outlet and analyzed offline using ImageJ® software. For E. coli BioParticles® and whole blood analysis, eluents were collected from the bacteria and waste outlets and separation efficiency was determined by flow cytometry (BD Accuri™ C6, BD Biosciences, USA).
Phd 2000
Manufactured by Harvard Apparatus
Sourced in United States, Canada, United Kingdom
The PHD 2000 is a programmable syringe pump that provides precision fluid control for laboratory applications. It offers a wide range of flow rates and can accommodate a variety of syringe sizes. The device is designed for ease of use and includes features such as a user-friendly interface and the ability to create customized dispensing programs.
Automatically generated - may contain errors
Lab products found in correlation
Novec 7500 engineered fluid, by 3M (2 mentions)
Pico surf, by Harvard Apparatus (2 mentions)
Labview 8, by National Instruments (2 mentions)
Te300, by Nikon (2 mentions)
Glass slide, by Thermo Fisher Scientific (2 mentions)
Glass tube, by World Precision Instruments (2 mentions)
Luer lok syringe, by BD (2 mentions)
Rnalater, by Thermo Fisher Scientific (2 mentions)
Adenosine, by Thermo Fisher Scientific (2 mentions)
Papaverine, by Merck Group (2 mentions)
Euthasol, by Virbac (2 mentions)
Axio observer microscope, by Zeiss (2 mentions)
Dmlb bright field microscope, by Leica (2 mentions)
Accuri c6, by BD (1 mentions)
Tapered capillary, by Zolix (1 mentions)
Stereotaxic apparatus, by RWD Life Science (1 mentions)
Ix71 inverted fluorescence microscope, by Olympus (1 mentions)
Sylgard 184, by Dow (1 mentions)
Recombinant human il 1β, by BioLegend (1 mentions)
Phantom v310, by Ametek (1 mentions)
157 protocols using phd 2000
1
Inertial Focusing of Bacteria and Blood Cells
2
Uniform High-Shear Blood Flow Device
3
Microfluidic Fabrication of GelMA Microgels
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A step emulsification microfluidic device was fabricated for high throughput microgel production as described previously 72 (link),73 (link). For soft or stiff GelMA microgels, 50 mg mL−1 or 150 mg mL−1 of GelMA solution was prepared in 01% w/v photoinitiator solutions, respectively. Novec™ 7500 engineered fluid (3M, MN, USA) supplemented with Pico-surf™ (2% v/v) (Sphere Fluidics, Cambridge, UK) was prepared for the continuous phase. The GelMA and Pico-surf™ solutions were then injected into the microfluidic device using syringe pumps (PHD 2000, Harvard Apparatus, MA, USA). The temperature was maintained at 35–40 °C using a space heater. Once collected in microcentrifuge tubes, microgel suspensions were stored at 4 °C to physically crosslink.
4
Hypoxia-Evoked Locomotory Response in C. elegans
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To examine hypoxia-evoked locomotory response, OP50 was seeded on 5.5 cm assay plates. Bacteria were grown for 16 hours, and lawn border was removed before use. 25–30 day-one adults were transferred to assay plates and sealed in a microfluidic chamber. The defined gases were delivered into the microfluidic device with a flow rate of 3 ml/min by a syringe pump (PHD2000, Harvard Apparatus). The rapid switch between 7% and 1% O2 was operated using Telfon valves (AutoMate Scientific) under the control of ValveBank Perfusion Controller. Videos were acquired with a FLIR camera mounted on a Zeiss Stemi-508 scope, and analyzed with a home-made MatLab program Zentracker [https://github.com/wormtracker/zentracker ].
5
Electrospinning of CS/PEO Nanofibers
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CS/PEO nanofibers were prepared according to Pakravan et al. (2011 ) using the electrospinning process. Electrospinning of the blend solution was performed using a horizontal homemade setup containing (1) a high voltage power supply (Gamma High Voltage Research, FL, USA), (2) a programmable pump (Harvard Apparatus, PHD 2000) to deliver the polymer solution at the required flow rate, and (3) a metallic rotating drum wrapped with an aluminum foil to collect the nanofibers. A schematic representation of the set up is shown in Figure 1 . The electrospinning blend solution was poured into a 10 ml syringe with Luer–Lock connection to an 18‐gauge blunt tip needle (Cadence Science, USA). The syringe was mounted on the pump with a grip and grounded by use of an alligator clip. The optimal process parameters were flow rate of 0.5 ml/hr, voltage of 20 kV, and needle tip‐to‐collector distance of 15 cm. All experiments were conducted at room temperature (22 ± 1°C), relative humidity of 20%, and under atmospheric pressure. The collected nanofibers were dried overnight under a hood to ensure complete evaporation of the solvent.
6
Microfluidic PDMS Chip Fluid Dynamics
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Fluid dynamic fields of fluid velocity around the fiber-shaped pillars, inside microfluidic PDMS chips, were observed by μPIV imaging. The 2D μPIV system (TSI Incorporated, Minneapolis, USA), composed by an inverted microscope (Olympus IX71), a laser (Nd:YAG 532 nm), and a camera (Power View 4M, 2048 9 2048 pixels), allowed calculation of the velocity field based on the correlation of fluorescent images, acquired with a known and proper time lapse (Δt). Excited by the laser, light emitted by fluorescent tracer particles (Øp = 1 µm, Thermo Fisher Scientific, Waltham, MA, USA, 1% solids, re-suspended 1:10 in distillated water solution) was recorded by the synchronized camera and elaborated by means of a dedicated algorithm.
The experimental setup was composed by a syringe pump (PHD2000, Harvard Apparatus, Holliston, MA, USA), a 5 ml glass syringe (Gastight Syringes, Hamilton Bonaduz AG, Switzerland) with a 18 G needle connected with PTFE tubing, that was then directly inserted into the PDMS test chamber. 70 couples of images (10x magnification) for each field of observation were elaborated to build each flow field (calibration of 0.46 µm/px, interrogation areas 32 x 32 pixel). Acquisition time was tuned from 250 to 2300 μs, in order to catch the range of velocity up to 7.5 mm/s.
The experimental setup was composed by a syringe pump (PHD2000, Harvard Apparatus, Holliston, MA, USA), a 5 ml glass syringe (Gastight Syringes, Hamilton Bonaduz AG, Switzerland) with a 18 G needle connected with PTFE tubing, that was then directly inserted into the PDMS test chamber. 70 couples of images (10x magnification) for each field of observation were elaborated to build each flow field (calibration of 0.46 µm/px, interrogation areas 32 x 32 pixel). Acquisition time was tuned from 250 to 2300 μs, in order to catch the range of velocity up to 7.5 mm/s.
7
Droplet Manipulation Robotic System
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The droplet robot was assembled with four parts: a high-precision syringe pump (PHD 2000, Harvard Apparatus, Holliston, USA) with a 1-μL syringe (7000 series, Hamilton, Reno, USA) for liquid metering; a tapered capillary (150-μm i.d., 250-μm o.d., Reafine Chromatography, Handan, China) was used to aspirate and dispense droplets; a high-density nanowell-array chip with a frame for droplet storage and incubation; an x-y-z translational stage (PSA series, Zolix, Beijing, China) was used to precisely control the movement of the microchip and 384-well plates relative to the tapered capillary. The capillary was connected with the 1-μL syringe fixed on the syringe pump via a Tygon tube. The syringe pump and the x-y-z translational stage were synchronously controlled using a program written with Labview (Labview 8.0, National Instruments, Austin, USA). Droplet manipulation process was monitored and recorded with a stereomicroscope (SMZ 850T, Touptek, Hangzhou, China) equipped with a CCD camera (HV3151UC, Daheng Imavision, Beijing, China). The syringe pump was operated with a flow rate of 200 nL/min under volume mode. The x-y-z translational stage was set at an initial velocity of 10 mm/s, an acceleration of 30 mm/s2, and an uniform velocity of 30 mm/s.
8
Ultrasound-Mediated Thrombolysis in Phantom
9
Microfluidic Droplet Generation via Photopolymerization
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Microfluidic devices were fabricated by thiol-ene closed-face photopolymerization as previously described, 45 placing optical adhesive NOA81 between two glass slides followed by UV-A exposure. 46 The channels were treated with a solution (10% v/v) of OTS in toluene and rinsed with toluene, isopropanol, ethanol and air dried before use. A flow-focussing geometry was used for emulsion droplet generation with microchannel dimensions: 400 mm height, 450 mm outlet channel width and a constriction width of 200 mm. The fluids were injected via syringe pumps (Harvard Apparatus PHD2000) with flow rates ranging from 1-100 mL min À1 . Water in oil (W/O) droplets and plugs were generated with 100-500 mm radii by controlling dispersed and continuous phase flow rates. The dispersed phase consisted of an aqueous PVA solution (E0.01-10% w/w) whilst the continuous phase comprised non-ionic surfactant Span s 80 (up to 2.5% v/v) in hexadecane, to prevent droplet coalescence at collection. Tubing attached to the outlet channel immersed the droplets produced into a large bath (25 mL) of non-solvent ethyl acetate.
10
Flow-Based Adhesion Assay for HUVEC Monolayers
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The protocols of the adhesion assays conducted under flow conditions were previously described in detail [14 (link)]. Briefly, HUVEC monolayers grown on coverslips were pretreated with 50 μM cholesterol or 7-ketocholesterol for 18 h, after which 0.1 ng/ml TNFα was added prior to an additional 4-h incubation. Next, the HUVECs were positioned in a flow chamber mounted on an inverted microscope (IX70, Olympus, Tokyo, Japan). The monolayers were perfused with perfusion medium for 5 min, after which the THP-1 cells (106/mL) were drawn through the chamber with a syringe pump (PHD2000, Harvard Apparatus) for 10 min at a controlled flow rate to generate a shear stress of 1.0 dyne/cm2. The entire period of perfusion was recorded on videotape, transferred to a personal computer and subjected to an image analysis to determine the numbers of rolling and adherent THP-1 cells on the HUVEC monolayers in 10 randomly selected 20 microscope fields.
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