A549 human lung adenocarcinoma cells

Manufactured by American Type Culture Collection

Sourced in United States

A549 human lung adenocarcinoma cells are a widely used cell line derived from a human lung adenocarcinoma. They are commonly used in research as a model for studying lung cancer and related cellular processes.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions) Dulbecco s modified eagle s medium, by Merck Group (3 mentions) Fetal bovine serum (fbs), by Merck Group (3 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (2 mentions) Doxorubicin hydrochloride powder, by Merck Group (2 mentions) Alamar blue 10x ready to use solution, by Merck Group (2 mentions) Hela human cervical cancer cell, by American Type Culture Collection (2 mentions) Dmem media, by Thermo Fisher Scientific (2 mentions) Pen strep antibiotics, by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Hek293 human embryonic kidney cells, by American Type Culture Collection (1 mentions) Pc 12 rat pheochromocytoma cells, by American Type Culture Collection (1 mentions) H1299, by American Type Culture Collection (1 mentions) Analytical grade organic solvents, by Thermo Fisher Scientific (1 mentions) Gemcitabine, by LC Laboratories (1 mentions) Heparinized sprague dawley rat plasma, by Innovative Research (1 mentions) Crystal violet, by Merck Group (1 mentions) Celltiter blue cell viability assay kit, by Promega (1 mentions) Pluronic p85, by BASF (1 mentions)

27 protocols using a549 human lung adenocarcinoma cells

Culturing Diverse Human and Rodent Cell Lines

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A549 human lung adenocarcinoma cells, H1299 human non-small cell lung carcinoma cells, HeLa human cervical cancer cells, NT2 human teratocarcinoma cells, and HEK293 human embryonic kidney cells were obtained from ATCC (Rockville, MD, USA) and grown in Dulbecco’s modified Eagles medium (DMEM) supplemented with 10% FBS and penicillin/streptomycin in a humidified atmosphere with 5% CO₂. HL-60 human promyelocytic leukemia cells and PC-12 rat pheochromocytoma cells were obtained from ATCC (Rockville, MD, USA) and grown in RPMI-1640 medium supplemented with 10% FBS and penicillin/streptomycin or RPMI-1640 medium supplemented with 10% horse serum, 5% FBS, and penicillin/streptomycin. 3T3-L1 mouse fibroblasts were obtained from the Japanese Collection of Research Bioresource Bank (Tokyo, Japan) and grown in DMEM supplemented with 10% bovine calf serum and penicillin/streptomycin.

Kamada R., Tano F., Kudoh F., Kimura N., Chuman Y., Osawa A., Namba K., Tanino K, & Sakaguchi K. (2016). Effective Cellular Morphology Analysis for Differentiation Processes by a Fluorescent 1,3a,6a-Triazapentalene Derivative Probe in Live Cells. PLoS ONE, 11(8), e0160625.

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Nanoparticle-based Cancer Drug Delivery

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All chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used as received. Analytical grade organic solvents and all other reagents were purchased from Fisher Scientific (Pittsburgh, PA, USA). Gemcitabine was purchased from LC Laboratories (Woburn, MA, USA). PEG-b-PLA was purchased from Advanced Polymer Materials Inc. (Montreal, Canada): the M_n of PEG and PLA was 4000 and 2200 g/mol, respectively; polydispersity index (PDI) 1.05. A549 human lung adenocarcinoma cells and PANC-1 pancreatic adenocarcinoma cells were purchased from ATCC (Manassas, VA, USA) and cultured in RPMI 1640 medium and Dulbecco’s modified Eagle’s medium (DMEM), respectively, supplemented with 10% fetal bovine serum, 100 IU/mL penicillin, 100 μg/mL streptomycin, and 2 mM L-glutamine in a 5% CO₂ incubator at 37 °C. Heparinized Sprague−Dawley rat plasma was purchased from Innovative Research Inc. (Novi, MI, USA).

Tam Y.T., Huang C., Poellmann M, & Kwon G.S. (2018). Stereocomplex Prodrugs of Oligo(lactic acid)n‑Gemcitabine in Poly(ethylene glycol)-block-poly(D,L‑lactic acid) Micelles for Improved Physical Stability and Enhanced Antitumor Efficacy. ACS nano, 12(7), 7406-7414.

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Culturing Diverse Cell Lines

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HepG2 human liver hepatocellular carcinoma cells, RAW264.7 murine macrophage-like cells, and A549 human lung adenocarcinoma cells were purchased from ATCC. They were cultured at 37 °C and 5% CO2 in RPMI1640, DMEM or F-12 K media, respectively, all supplemented with 10% FBS and 1% penicillin/streptomycin. Media and supplements were purchased from Corning Cellgro (Mediatech, Manassas, VA).

Moerland J.A., Zhang D., Reich L.A., Carapellucci S., Lockwood B., Leal A.S., Krieger-Burke T., Aleiwi B., Ellsworth E, & Liby K.T. (2020). The novel rexinoid MSU-42011 is effective for the treatment of preclinical Kras-driven lung cancer. Scientific Reports, 10, 22244.

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Anticancer Activity of (−)-Gossypol

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(−)-gossypol was a kind gift from Dr. Michael Dowd (U.S. Department of Agriculture, Washington D.C.) Pluronic P85 (P85) was kindly provided by BASF Corp. (Florham Park, NY). A549 human lung adenocarcinoma cells were purchased from ATCC (Manassas, VA). CellTiter-Blue® Cell Viability Assay kit was purchased from Promega (Madison, WI). Crystal violet was purchased from Sigma-Aldrich (St. Louis, MO). All other reagents were obtained from Thermo Fisher Scientific Inc. (Fairlawn, NJ) and were of analytical grade.

Tomoda K., Chiang H.C., Kozak K.R, & Kwon G.S. (2016). Injectable (−)-Gossypol-loaded Pluronic P85 Micelles for Cancer Chemoradiotherapy. International journal of radiation biology, 93(4), 402-406.

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Cytotoxicity Assay in A549 Cells

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A549 human lung adenocarcinoma cells with the alveolar type II phenotype were obtained from ATCC (Manassas, VA, USA). Doxorubicin hydrochloride ® powder (Sigma Life Sciences, Ireland) was diluted in1mL sterile water to the required concentration (17.25 mM).
Alamar blue (AB) (10X ready to use solution) and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) were obtained from Sigma Aldrich, Ireland.
For cytotoxicity assays, an AB/MTT solution, 1.5mL of AB and 3 mL of MTT stock solution (2.5mg/mL, 25mg MTT/10mL PBS) in 30mL of fresh medium was prepared prior to the tests.

Farhane Z., Bonnier F., Howe O., Casey A, & Byrne H.J. (2018). Doxorubicin kinetics and effects on lung cancer cell lines using in vitro Raman micro-spectroscopy: binding signatures, drug resistance and DNA repair. Journal of biophotonics, 11(1).

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Cytotoxicity Assay in A549 Cells

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Farhane Z., Bonnier F, & Byrne H.J. (2017). Monitoring doxorubicin cellular uptake and trafficking using in vitro Raman microspectroscopy: short and long time exposure effects on lung cancer cell lines. Analytical and bioanalytical chemistry, 409(5).

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Culturing A549 Lung Cancer Cells

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A549 human lung adenocarcinoma cells were purchased from American Tissue Culture Collection (Manassas, VA, U.S.A.) and maintained in Dulbecco’s modified Eagle medium supplemented with 10% heat-inactivated fetal bovine serum (GIBCO, NY, U.S.A.) at 37°C in a humid 5% CO₂ atmosphere. The proteasome inhibitor MG132 (carbobenzoxy-L-leucyl-L-leucyl-L-leucinal) and protein synthesis inhibitor cycloheximide (CHX) were purchased from Calbiochem (Darmstadt, Germany) and Sigma–Aldrich (MO, U.S.A.) respectively. Protein G dynabeads for immunoprecipitation was purchased from ThermoFisher Scientific (MA, U.S.A.). D-luciferin and coelenterazine-h were purchased from Promega (WI, U.S.A.).

Kim Y.E., Choi S., Kim J.O, & Kim K.K. (2017). RBFOX3 regulates Claudin-1 expression in human lung tissue via attenuation of proteasomal degradation. Bioscience Reports, 37(1), BSR20160623.

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TGF-β Modulation in A549 Cells

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A549 human lung adenocarcinoma cells were purchased from American Tissue Culture Collection (USA). They were grown in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Gibco, USA) at 37°C in a humid 5% CO₂ atmosphere. Neutralizing anti-TGF-β antibody (1D11) and human TGF-β1 (R&D Systems, USA) were supplemented into the culture medium at concentrations of 25 μg/ml and 5 ng/ml, respectively. Transfection was performed using the PolyMAG Magnetofection kit (Chemicell GmbH, Germany) according to the manufacturer’s instructions.

Kim Y.E., Kim J.O., Park K.S., Won M., Kim K.E, & Kim K.K. (2016). Transforming Growth Factor-β-Induced RBFOX3 Inhibition Promotes Epithelial-Mesenchymal Transition of Lung Cancer Cells. Molecules and Cells, 39(8), 625-630.

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Adenovirus Cell Culture Protocol

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A549 human lung adenocarcinoma cells were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA), while HEK293 human embryonic kidney cells were purchased from Microbix (Mississauga, ON, Canada). Both cell lines were cultured in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich, St Louis, MO, USA) with 10% fetal bovine serum (FBS) at 37 °C. The Ad5 isolate we used is named Ad5 wt500, and it was derived by plaque purification by our laboratory from a wild-type Ad5 stock purchased from ATCC. A human Ad6 isolate (Tonsil 99; VR-6) was purchased from ATCC and cultured and purified as described previously [33 (link)]. The titer of the virus stocks was determined by plaque assay [33 (link)].

Toth K., Spencer J.F., Ying B., Tollefson A.E, & Wold W.S. (2017). HAdV-C6 Is a More Relevant Challenge Virus than HAdV-C5 for Testing Antiviral Drugs with the Immunosuppressed Syrian Hamster Model. Viruses, 9(6), 147.

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Culture of A549 Lung Cancer Cells

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A549 human lung adenocarcinoma cells were purchased from the American Type Culture Collection (ATCC). Cells were cultured in Dulbecco’s Modified Eagle Medium (Invitrogen Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS, Sigma–Aldrich, St. Louis, MO, USA) and 1% penicillin-streptomycin (Invitrogen) under a humidified environment with 5% CO₂ at 37 °C.

Luo D., Putra M.Y., Ye T., Paul V.J, & Luesch H. (2019). Isolation, Structure Elucidation and Biological Evaluation of Lagunamide D: A New Cytotoxic Macrocyclic Depsipeptide from Marine Cyanobacteria. Marine Drugs, 17(2), 83.

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