Igr39

Human colon adenocarcinoma cell line (HT-29), human malignant melanoma cell line (IGR39), human clear renal carcinoma cell line (CaKi-1), and human endothelial cells (EC) CRL-1730 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). The cell culture medium consisted of DMEM Glutamax medium supplemented with 10% FBS and 1% antibiotics. Cells were kept at 37 °C with 5% CO₂ in a humidified atmosphere and were grown to 70% confluence. During the passage, cells were trypsinized with 0.125% TrypLE™ Express solution and used till passage 20.

The human malignant melanoma cell line IGR39, human triple-negative breast cancer MDA-MB-231, and human pancreatic carcinoma cell line Panc-1 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). Human foreskin fibroblasts (HF) CRL-4001 were originally obtained from ATCC and kindly provided by Prof. Helder Santos (University of Helsinki, Finland). Primary prostate carcinoma PPC-1 cell line was kindly provided by Prof. Tambet Teesalu (University of Tartu, Estonia). All cell lines were cultured in Dulbecco’s Modified Eagle’s GlutaMAX medium (Gibco (Carlsbad, CA, USA)), supplemented with 10,000 U/mL penicillin, 10 mg/mL streptomycin (Gibco), and 10% fetal bovine serum (Gibco). Cell cultures were grown at 37 °C in a humidified atmosphere containing 5% CO₂. They were used until the passage of 20.