Human OS cell lines HOS-MNNG and U2OS were obtained from the Cell Line Service (Eppelheim, Baden-Württemberg, Germany). HOS-143B, MG63 and Saos-2 were obtained from the American Type Culture Collection (ATCC, Manassas, VA). Primary OS cells were generated and extracted following previously described protocol [15, 16] . The MNNG-HOS cells were grown in the Roswell Park Memorial Institute (RPMI). The MG63, and primary cells were grown in Dulbecco's modi ed Eagle's medium (DMEM). U2OS and Saos-2 cells were cultured in Dulbecco's Modi ed Eagle Medium/Nutrient Mixture F-12 medium (DMEM-F12). HOS-143B was cultured in DMEM-Brdu. All cell lines and primary cells were cultured in 10% (v/v) fetal bovine serum and maintained in a humidi ed atmosphere of 37°C with 5% CO 2 [15] .
Hos 143b
Manufactured by American Type Culture Collection
The HOS-143B is a laboratory equipment product designed for cell culture applications. It features a built-in incubator and shaker mechanism for maintaining optimal conditions for cell growth and proliferation. The device is capable of precisely controlling temperature, humidity, and agitation speed to support a wide range of cell lines and experimental protocols.
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Lab products found in correlation
U2 os, by Cytion (1 mentions)
Saos 2, by American Type Culture Collection (1 mentions)
Cariporide, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Hfob1, by American Type Culture Collection (1 mentions)
2 protocols using hos 143b
1
Osteosarcoma Cell Line Cultivation Protocol
2
Oxidative Stress Response in Osteosarcoma Cells
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The human OS cell lines U2OS, HOS, HOS-143B, and KHOS as well as the osteoblast cell line hFOB 1.19 were purchased from American Type Culture Collection (Rockville, MD). The cells were cultured in DMEM medium (Gibco/Life Technologies, Carlsbad, CA) supplemented with 10% fetal bovine serum (Gibco/Life Technologies). Cells were grown under standard culture conditions (37 °C, humidified atmosphere composed of 95% air and 5% CO2).
U2OS cells were incubated with tert-butyl hydroperoxide (tBHP, 1–100 μM, Sigma-Aldrich), the antioxidant 1,3-dimethyl-2-thiourea (DMTU, 30 mM, Sigma-Aldrich), or the specific NHE1 inhibitor cariporide (10 μM, Sigma-Aldrich) that had been added to serum-free medium directly before incubation with cells. cariporide was dissolved in dimethyl sulfoxide (DMSO, final concentration of 0.1%).
For the ROS and Western blot assays, the cells were harvested after 4 h incubation. For the cell viability assays, cells were harvested at the indicated time points after incubation. For cell cycle and apoptosis assays, cells were harvested after 24 h incubation.
U2OS cells were incubated with tert-butyl hydroperoxide (tBHP, 1–100 μM, Sigma-Aldrich), the antioxidant 1,3-dimethyl-2-thiourea (DMTU, 30 mM, Sigma-Aldrich), or the specific NHE1 inhibitor cariporide (10 μM, Sigma-Aldrich) that had been added to serum-free medium directly before incubation with cells. cariporide was dissolved in dimethyl sulfoxide (DMSO, final concentration of 0.1%).
For the ROS and Western blot assays, the cells were harvested after 4 h incubation. For the cell viability assays, cells were harvested at the indicated time points after incubation. For cell cycle and apoptosis assays, cells were harvested after 24 h incubation.
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