The LDH release, GSH content, caspase-3 activity and caspase-9 activity were determined using the LDH cytotoxicity kit, glutathione reductase assay kit, caspase-3 activity assay kit and caspase-9 activity assay kit (Nanjing JianCheng Bio Institute, Nanjing, China), respectively, according to the manufacturer protocol [18 (link),27 (link),28 (link)].
Ldh cytotoxicity kit
Manufactured by Nanjing Jiancheng
Sourced in China
The LDH cytotoxicity kit is a laboratory reagent used to measure the release of lactate dehydrogenase (LDH) from cells. LDH is an enzyme found in the cytoplasm of cells and is released when cells are damaged or undergoing cell death. The kit provides a colorimetric assay to quantify the amount of LDH released, which can be used as an indicator of cytotoxicity or cell viability.
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2 protocols using ldh cytotoxicity kit
1
Assaying Cellular Cytotoxicity and Apoptosis
2
LDH Activity Measurement in Oxidative Stress
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The LDH activity of the cell supernatant in the Se cytotoxicity assay and oxidative stress model was determined using an LDH Cytotoxicity kit (Jiancheng Bioengineering Institute, Nanjing, China). Briefly, after exposure to 1000 μmol/L H2O2 and with/without 100 ng/mL Se for 24 h, a combination of 120 μL cell-free supernatant and 60 μL LDH reaction mixture was incubated at room temperature in the dark for 30 min. The cell-free supernatant of cells without the H2O2 and Se treatments served as the negative control. The absorbance was detected at a wavelength of 490 nm.
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