3 morpholinopropane 1 sulfonic acid mops

Manufactured by Nacalai Tesque

Sourced in Japan

3-morpholinopropane-1-sulfonic acid (MOPS) is a chemical compound used as a buffer in biological and biochemical applications. It maintains a stable pH environment in aqueous solutions, typically in the range of 6.5 to 8.5.

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Lab products found in correlation

N decane, by Fujifilm (3 mentions) Potassium chloride kcl, by Nacalai Tesque (3 mentions) Milli q system, by Merck Group (3 mentions) 1 2 dioleoyl sn glycero 3 phosphoethanolamine dope, by Avanti Polar Lipids (2 mentions) 1 2 dioleoyl sn glycero 3 phospho 1 rac glycerol dopg, by Avanti Polar Lipids (2 mentions) 1 2 dioleoyl sn glycero 3 phosphocholine dopc, by Avanti Polar Lipids (2 mentions) Cecropin a, by Bachem (1 mentions) Ll 37, by AnaSpec (1 mentions) Sucrose, by Fujifilm (1 mentions) Liquid paraffin, by Fujifilm (1 mentions) Glucose, by Fujifilm (1 mentions) Calcein, by Merck Group (1 mentions) 1 2 dioleoyl sn glycero 3 phosphoethanolamine n, by Avanti Polar Lipids (1 mentions) Rhodamine pe, by Avanti Polar Lipids (1 mentions) Potassium chloride (kcl), by Merck Group (1 mentions) Hexadecane, by Fujifilm (1 mentions) 1 2 diphytanoyl sn glycero 3 phosphocholine dphpc, by Avanti Polar Lipids (1 mentions) Milli q, by Merck Group (1 mentions) Su 8 3025, by MicroChem (1 mentions) N decane, by Merck Group (1 mentions)

5 protocols using 3 morpholinopropane 1 sulfonic acid mops

Optimizing Lipid Membrane Composition for Antimicrobial Peptide Characterization

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In this study, we used the following reagents: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE; Avanti Polar Lipids,
AL, USA); 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DOPG; Avanti Polar Lipids); n-decane (Wako Pure Chemical Industries, Ltd., Osaka, Japan); 3-morpholinopropane-1-sulfonic
acid (MOPS, Nacalai Tesque, Kyoto, Japan); potassium chloride (KCl;
Nacalai Tesque); clavanin A (GenScript); cecropin A (BACHEM); pardaxin
(abcam); magainin 1 (AnaSpec Inc); SL-37 (Cosmo Bio Co., Ltd.); and
LL-37 (AnaSpec Inc.). DOPE and DOPG were melted in n-decane at a concentration of 10 mg/mL, and we obtained a lipid mixture
of DOPE/DOPG (3:1 mol/mol). The composition of the measurement buffer
was regulated so that it became 200 mM KCl, 10 mM MOPS, and pH 7.0
in the Milli-Q system (Millipore, Billerica, MA, USA). The clavanin
A, cecropin A, pardaxin, magainin 1, SL-37, and LL-37 powders were
dissolved in the measurement buffer and preserved at 4 °C.

Saigo N., Izumi K, & Kawano R. (2019). Electrophysiological Analysis of Antimicrobial Peptides in Diverse Species. ACS Omega, 4(8), 13124-13130.

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Lipid Vesicle Formation with Peptides

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In this study, the following chemicals were used: 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC; Avanti Polar Lipids, Birmingham, AL, USA), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhodamine PE; Avanti Polar Lipids, Birmingham, AL, USA), liquid paraffin (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), calcein (Sigma-Aldrich Co, LCC., St. Louis, MO, USA), glucose (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), sucrose (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), n-decane (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 3-morpholinopropane-1-sulfonic acid (MOPS, Nacalai Tesque, Kyoto, Japan), potassium chloride (KCl, Nacalai Tesque, Kyoto, Japan). As peptides, we used C105Y (GenScuript, Piscataway, NJ, USA), melittin (synthesized and purified as powder), ovispirin (KareBay Biochem, Inc., Monmouth Junction, NJ, USA), and TAT (BACHEM, Bubendorf, Switzerland). Peptides were stored at −20 °C. For use, samples were diluted to their designated concentration using a buffered electrolyte solution and stored at 4 °C.

Izumi K., Saito C, & Kawano R. (2023). Liposome Deformation Induced by Membrane-Binding Peptides. Micromachines, 14(2), 373.

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Single-Molecule Nanopore Measurements

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In this study, we used the following
reagents: 1,2-diphytanoyl-sn-glycero-3-phosphocholine
(DPhPC; Avanti. Polar Lipids, Alabaster, Alabama), n-decane (Wako Pure Chemical Industries, Ltd., Osaka, Japan), potassium
chloride (KCl; Nacalai Tesque), 3-morpholinopropane-1-sulfonic acid
(MOPS; Nacalai Tesque, Kyoto, Japan). Buffered electrolyte solutions
(1 M KCl, 10 mM MOPS, pH 7.0) were prepared using ultrapure water,
which was obtained from a Milli-Q system (Millipore, Billerica, Massachusetts).
Wild-type α-hemolysin (αHL; Sigma-Aldrich, St. Louis,
Missouri) was obtained as the monomer polypeptide, isolated from Staphylococcus aureus in the form of a powder and
dissolved at a concentration of 1 mg/mL in ultrapure water. For use,
samples were diluted to the designated concentration using a buffered
electrolyte solution and stored at 4 °C. HPLC-grade DNA, RNA,
and LNA-inserted DNA oligonucleotides were synthesized by Eurofins
Genomics Inc. (Tokyo, Japan), FASMAC Co., Ltd. (Kanagawa, Japan),
and GeneDesign, Inc. (Osaka, Japan), respectively, stored at −20
°C. 10× TBE buffer was obtained from Takara Bio Inc. (Shiga,
Japan) and was 10-fold diluted for gel electrophoresis. The power
supply and an LED transilluminator were obtained from Bio Craft Co.,
Ltd. (Tokyo, Japan) and ATTO Co., Ltd. (Tokyo, Japan), respectively.

Takiguchi S., Kambara F., Tani M., Sugiura T, & Kawano R. (2023). Simultaneous Recognition of Over- and Under-Expressed MicroRNAs Using Nanopore Decoding. Analytical Chemistry, 95(39), 14675-14685.

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Multicomponent Lipid Bilayer Assembly

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1,2-Diphytanoyl-sn-glycero-3-phosphocholine (DPhPC; Avanti Polar Lipids, Alabaster, AL, USA) and hexadecane (Wako Pure Chemical Industries, Ltd., Osaka, Japan) were used as the lipid/oil solution. Potassium chloride (KCl; Nacalai Tesque, Kyoto, Japan) and 3-morpholinopropane-1-sulfonic acid (MOPS; Nacalai Tesque, Kyoto, Japan) were used as the aqueous solution (1.0 M KCl, 10 mM MOPS, pH 7.0). The MOPS solution was prepared using ultrapure water from a Milli-Q (Merck Millipore Corp., Billerica, MA, USA). Alpha-hemolysin (αHL; Sigma-Aldrich, St. Louis, MO, USA) was obtained as a monomer protein isolated from Staphylococcus aureus in the form of a powder, dissolved at a concentration of 1 mg/mL in ultrapure water, and stored at −80 °C. For use, samples were diluted in the designated concentration using a buffered electrolyte solution and stored at 4 °C. SU-8 3025 (MicroChem Corp., Newton, MA, USA) was used as a photoresist. Polydimethylsiloxane (PDMS; Sylgard 184, Dow Corning Toray Co., Ltd., Tokyo, Japan) was used for the material of microchannels.

Shoji K, & Kawano R. (2018). Microfluidic Formation of Double-Stacked Planar Bilayer Lipid Membranes by Controlling the Water-Oil Interface. Micromachines, 9(5), 253.

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Magainin 1 Peptide Characterization

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The reagents used in this study were as follows: 1,2-dioleoylsn-glycero-3-phosphocholine (DOPC, Avanti Polar Lipids, Alabaster, AL); 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE, Avanti Polar Lipids); 1,2-dioleoyl-sn-glycero-3phospho-(1′-rac-glycerol) (DOPG, Avanti Polar Lipids); n-decane (Sigma-Aldrich, St. Louis, MO); 3-morpholinopropane-1-sulfonic acid (MOPS, Nacalai Tesque, Kyoto, Japan); and KCl (Nacalai Tesque). Buffered electrolyte solutions were prepared from ultrapure water, which was obtained from a Milli-Q system (Millipore, Billerica, MA). Magainin 1 (LKT Laboratories Inc., St. Paul, MN) was obtained as a monomer polypeptide isolated from African frogs in the form of a powder, and dissolved at a concentration of 1 mM in ultrapure water. For use, samples were diluted to 10 μM using a buffered electrolyte solution and stored at 4°C.

Watanabe H, & Kawano R. (2016). Channel Current Analysis for Pore-forming Properties of an Antimicrobial Peptide, Magainin 1, Using the Droplet Contact Method. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 32(1).

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