EEG data were collected using a vertical one-channel montage configuration, using three electrodes, in which active and reference electrodes were placed on the mastoids, and a ground electrode was placed on the forehead. A researcher applied 1′′ square cloth solid gel electrodes (EL504, BIOPAC Systems, Inc.) to the mastoids and forehead; electrodes were connected to a BIOPAC MP150 data acquisition system and ERS100C Evoked Response Amplifier (BIOPAC Systems, Inc.). Data were recorded at a sampling rate of 20 kHz, with an online low-pass filter of 10 kHz and a high-pass filter of 1 Hz; the signal was recorded using Acknowledge software (AcqKnowledge, version 4.1). Stimuli were presented for 25 minutes in total, during which time participants shown a silent film2 , to promote relaxation and stillness during the EEG.
Ers100c evoked response amplifier
Manufactured by Biopac
The ERS100C Evoked Response Amplifier is a laboratory equipment designed to record and amplify evoked responses, such as visual, auditory, or somatosensory responses. It features adjustable gain and filtering options to enhance the signal-to-noise ratio of the recorded data.
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2 protocols using ers100c evoked response amplifier
1
Resting-State EEG Recording Protocol
2
Evaluating Retinal Function Post-Treatment
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ERG was performed 7 days after treatment. The rats were first adapted by keeping them for 30 minutes in the dark. Subsequently, the animals were anesthetized and corneal silver electrode was placed on the cornea, the reference needle electrode (#EL452, Biopac System, Goleta, CA, USA) was placed subcutaneously in the region of the skull and ground needle electrode (#EL450, Biopac System) was placed subcutaneously at the base of the tail (Shabelnikova et al., 2016). A stroboscope TSD122B with a flash of white light connected to the stimulator (#STM200, Biopac System) was placed in front of the animal. Evoked biopotentials were amplified using ERS100C Evoked Response Amplifier (#ERS100C, Biopac System). An MP150 data acquisition and analysis system (#MP150, Biopac Systems) was used to represent them graphically. Each rat was subjected to only a single stimulation of 0.5 seconds to obtain the ERG recording. Changes in the amplitudes of the a- and b-waves of ERG were used to assess the functional state of the retina after pre-treatment with MgAT and NMDA exposure. The readings from each group of eight animals were included into the protocol and used to calculate the mean.
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