Anti non phospho active β catenin ser33 37 thr41

Manufactured by Cell Signaling Technology

Anti-non-phospho (Active) β-catenin (Ser33/37/Thr41) is a primary antibody that specifically recognizes the non-phosphorylated, active form of β-catenin at serine 33, 37, and threonine 41 residues.

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Lab products found in correlation

Anti phospho histone h3 ser10, by Cell Signaling Technology (1 mentions) Anti phospho β catenin ser552, by Cell Signaling Technology (1 mentions) Ecl prime western blotting detection reagent, by GE Healthcare (1 mentions) Geliance 600 imaging system, by PerkinElmer (1 mentions) Nucleospin rna protein kit, by Macherey-Nagel (1 mentions) Anti β actin, by Abcam (1 mentions) Anti phospho β catenin ser33 37 thr41, by Cell Signaling Technology (1 mentions) Anti c myc 10828 1 ap, by Proteintech (1 mentions) Anti phosphotyrosine, by Merck Group (1 mentions) Anti β trcp, by Cell Signaling Technology (1 mentions) Anti β catenin 51067 2 ap, by Proteintech (1 mentions) Anti acetyl histone h3, by Cell Signaling Technology (1 mentions) Anti phospho threonine, by Cell Signaling Technology (1 mentions) Anti cyclin d1 60186 1 ig, by Proteintech (1 mentions)

2 protocols using anti non phospho active β catenin ser33 37 thr41

Protein Expression and Cell Cycle Analysis

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Total protein was extracted from NE-4C cells using the NucleoSpin RNA/Protein Kit (Macherey-Nagel). Samples were separated by polyacrylamide gel electrophoresis (PAGE). Primary antibodies used for EP expression levels include rabbit polyclonal anti-EP1, −EP2, −EP3, −EP4 (1:200; Santa Cruz Biotechnology). Detection of rabbit monoclonal anti-Phospho-Histone H3 (Ser10) (1:1000; Cell Signaling) was used as a measure of cell splitting behaviour. Primary antibodies used for β-catenin expression levels were rabbit monoclonal anti-non-phospho (Active) β-catenin (Ser33/37/Thr41) and rabbit polyclonal anti-phospho-β-catenin (Ser552) (1:1000; Cell Signaling). Blots were reprobed with mouse monoclonal anti-β-Actin (1:10,000; Abcam). Visualization of bound anti-rabbit and anti-mouse horseradish peroxidise-conjugated secondary antibodies was achieved by incubation with ECL Prime Western Blotting Detection Reagent (GE Healthcare) and detection by Geliance 600 Imaging System (Perkin Elmer).

Wong C.T., Ahmad E., Li H, & Crawford D.A. (2014). Prostaglandin E2 alters Wnt-dependent migration and proliferation in neuroectodermal stem cells: implications for autism spectrum disorders. Cell Communication and Signaling : CCS, 12, 19.

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Profiling β-catenin Signaling Pathway

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Immunoprecipitation and Western blotting were performed as described previously.^{35 (link)} GAPDH protein levels were used as loading controls. We used the following primary antibodies: anti-Non-phospho (Active) β-catenin (Ser33/37/Thr41) (8814, Cell Signaling Technology), anti-phospho-β-catenin (Ser33/37/Thr41) (9561, Cell Signaling Technology), anti-β-catenin (51067-2-AP, Proteintech), anti-β-TrCP (4394, Cell Signaling Technology), anti-FAM83A (A15201, ABclonal), anti-c-myc (10828-1-AP, Proteintech), anti-CyclinD1 (60186-1-Ig, Proteintech), anti-AXIN2 (20540-1-AP, Proteintech), anti-mouse HA (M180-3, EMD Millipore), anti-Rabbit HA (51064-2-AP, Proteintech), anti-mouse DYKDDDDK (M185, MBL), anti-Rabbit DYKDDDDK (80010-1-RR, Proteintech), anti-GAPDH (60004-1-Ig, Proteintech), anti-GFP (598, EMD Millipore), anti-phosphotyrosine (P4110, Sigma), anti-phosphoserine (05-1000x, EMD Millipore), anti-phospho-threonine (9386 S, Cell Signaling Technology), anti-GSK3β (22104-1-AP, Proteintech), anti-AXIN1 (16541-1-AP, Proteintech), anti-TCF4 (22337-1-AP, Proteintech), anti-BLK (10510-1-AP, Proteintech), anti-HDAC1 (10197-1-AP, Proteintech), anti-HDAC2 (12922-2-AP, Proteintech), anti- Acetyl-Histone H3 (8173, Cell Signaling Technology).

Zhou C., Zhu X., Liu N., Dong X., Zhang X., Huang H., Tang Y., Liu S., Hu M., Wang M., Deng X., Li S., Zhang R., Huang Y., Lyu H., Xiao S., Luo S., Ali D.W., Michalak M., Chen X.Z., Wang Z, & Tang J. (2023). B-lymphoid tyrosine kinase-mediated FAM83A phosphorylation elevates pancreatic tumorigenesis through interacting with β-catenin. Signal Transduction and Targeted Therapy, 8, 66.

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