Flag ulk1

Manufactured by Addgene

Flag-ULK1 is a recombinant protein that contains the ULK1 (Unc-51 Like Autophagy Activating Kinase 1) gene fused with a FLAG tag. ULK1 is a serine/threonine protein kinase that plays a key role in the initiation of autophagy, a cellular process that degrades and recycles damaged or unwanted cellular components.

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PCDNA3 Flag ULK1 (3 citations)

3 protocols using flag ulk1

Molecular Mechanisms of NRF2 Regulation

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GFP‐polyQ74 (#40262), GFP‐p62 (#38277), GFP‐NRF2 (#21549), GFP‐KEAP1 (#28025), Flag‐KEAP1 (#28023), Myc‐NRF2 (#21555), Flag‐NRF2 (#36971), HA‐K48 (#17605), HA‐K63 (#17606), Flag‐ULK1 (#27636) were procured from Addgene. GFP‐TRIM16 and Flag‐TRIM16 were cloned as described previously (Chauhan et al, 2016). Myc‐TRIM16, Myc‐TRIM16 deletion constructs, and His‐K63‐UB were generated using Gateway cloning strategy as per standard protocol (Invitrogen).
The siRNA for NRF2 and p62 were purchased from Sigma, and TRIM16, Ubb, Ube2n siRNA were from Dharmacon. For overexpression experiments, HEK293T cells were transfected using calcium phosphate method as per the manufacturer's instructions (Profection, Promega). Other cells are transfected using Effectene (Qiagen) or Viafect (Promega) or Interference (Polyplus) as per the manufacturer's instruction.

Jena K.K., Kolapalli S.P., Mehto S., Nath P., Das B., Sahoo P.K., Ahad A., Syed G.H., Raghav S.K., Senapati S., Chauhan S, & Chauhan S. (2018). TRIM16 controls assembly and degradation of protein aggregates by modulating the p62‐NRF2 axis and autophagy. The EMBO Journal, 37(18), e98358.

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Characterization of MAPK15 Mutants and Parkin-Related Constructs

Cited 3 times

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pCEFL‐HA‐MAPK15 and all its mutants were already described (Colecchia et al., 2012 (link); Iavarone et al., 2006 (link)). pCEFL‐MAPK15_WT IRES‐GFP and all its mutants (AXXA; KD) were previously described (Colecchia et al., 2015 (link)). MAPK15_WT, MAPK15_AXXA and MAPK15_KD mutants resistant to siRNA for MAPK15 were previously described (Rossi et al., 2016 (link)). pRK5‐Myc‐Parkin (MYC‐PRKN) (Addgene #17612), FLAG‐ULK1 (Addgene #24301) and mCherry‐Parkin (mCherry‐PRKN) (Addgene #23956), pHAGE‐mt‐mKeima (mt‐Keima) (Addgene #131626) were provided by Addgene as the kind gifts from the producing laboratories. The pDsRed2‐Mito plasmid was purchased from Clontech (Cat. #63242).

Franci L., Tubita A., Bertolino F.M., Palma A., Cannino G., Settembre C., Rasola A., Rovida E, & Chiariello M. (2022). MAPK15 protects from oxidative stress‐dependent cellular senescence by inducing the mitophagic process. Aging Cell, 21(7), e13620.

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Characterization of NRF2-KEAP1 Pathway

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GFP-polyQ74 (#40262), GFP-p62 (#38277), GFP-NRF2 (#21549), GFP-KEAP1 (#28025), Flag-KEAP1 (#28023), Myc-NRF2 (#21555), Flag-NRF2 (#36971), HA-K48 (#17605), HA-K63 (#17606), Flag-ULK1 (#27636) were procured from Addgene. GFP-TRIM16 and Flag-TRIM16 were cloned as described previously (Chauhan et al, 2016 (link)). Myc-TRIM16, Myc-TRIM16 deletion constructs, and His-K63-UB were generated using Gateway cloning strategy as per standard protocol (Invitrogen).
The siRNA for NRF2 and p62 were purchased from Sigma, and TRIM16, Ubb, Ube2n siRNA were from Dharmacon. For overexpression experiments, HEK293T cells were transfected using calcium phosphate method as per the manufacturer’s instructions (Profection, Promega). Other cells are transfected using Effectene (Qiagen) or Viafect (Promega) or Interference (Polyplus) as per the manufacturer’s instruction.

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